Automated methods and systems for washing platelet concentrates

Patent metadata

Field	Value
Publication number	US-2016333315-A1
Application number	US-201615193397-A
Country	US
Kind code	A1
Filing date	Jun 27, 2016
Priority date	Apr 7, 2011
Publication date	Nov 17, 2016
Grant date	—

Abstract

Automated systems and methods for providing platelet concentrates and synthetic storage media with reduced residual plasma volumes are disclosed. The disclosed systems and methods reduce the residual volume of plasma in platelet concentrate to obtain a platelet product having a volume of plasma that is approximately 5% or less of the total platelet product volume. The disclosed systems and methods also reduce the residual volume of plasma in platelet concentrate to obtain a washed platelet product, wherein the volume of plasma in the washed platelet product is approximately 1% or less of the total washed platelet product volume. Storage media for platelets including less than approximately 10% plasma are also disclosed.

First claim

1 - 23 . (canceled)

24 . An automated method of washing platelets comprising:
providing an automated separation device comprising a platelet concentration chamber, introducing a blood product including at least platelets and plasma into said chamber, separating said blood product to obtain a platelet concentrate, said platelet concentrate comprising platelets suspended in a residual volume of plasma, introducing a wash solution into said separation device, and removing residual plasma from said chamber to provide a washed platelet product comprising platelets, wash solution and plasma, wherein the volume of plasma in said washed platelet product is 1% or less of the total washed platelet product.

25 . The method of claim 24 comprising sequentially introducing two or more batches of said blood product wherein each sequential introduction is followed by said separation, said wash solution introduction and said removal step to arrive at said volume of plasma.

26 - 32 . (canceled)

33 . A method of washing platelets comprising:
providing an apheresis device, separating platelets suspended in plasma in said apheresis device to obtain concentrated platelets suspended in a reduced volume of plasma, collecting substantially all of said concentrated platelet suspension, removing any residual plasma remaining in said apheresis device, re-suspending said concentrated platelets in a volume of aqueous additive solution, wherein said aqueous additive solution comprises:
from about 45 to about 120 mM sodium chloride;
from about 5 to about 15 mM sodium citrate;
from about 20 to about 40 mM sodium acetate;
from about 0.05 to about 12 mM phosphate;
from about 0.05 to about 3 mM magnesium ion;
from about 0.05 to about 10 mM potassium chloride;
from about 5 to about 40 mM sodium bicarbonate; and
from about 0.5 to about 20 mM glucose; and
conveying said resuspended platelets into said apheresis device to remove additional plasma from said platelet suspension to provide a concentrated platelet product comprising platelets, said aqueous additive solution and plasma, wherein the volume of plasma in said platelet product is 5% or less of the total platelet product volume.

Assignees

• Fenwal Inc

Inventors

• Radwanski Katherine
• Min Kyungyoon

Classifications

• A61P7/00

  Drugs for disorders of the blood or the extracellular fluid · CPC title

• A61P7/04

  Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents · CPC title

• C12N5/0644 Primary

  Platelets; Megakaryocytes · CPC title

• A61M1/0272

  Apparatus for treatment of blood or blood constituents prior to or for conservation, e.g. freezing, drying or centrifuging · CPC title

• A61K35/19

  Platelets; Megacaryocytes · CPC title