Method of producing erythrocytes
US-9200253-B1 · Dec 1, 2015 · US
Novel Method for Producing Differentiated Cells
US2016115449A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016115449-A1 |
| Application number | US-201514925508-A |
| Country | US |
| Kind code | A1 |
| Filing date | Oct 28, 2015 |
| Priority date | Sep 15, 2009 |
| Publication date | Apr 28, 2016 |
| Grant date | — |
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Abstract
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The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.
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1 - 30 . (canceled) 31 . A method of producing erythroid cells comprising: forcibly expressing MYC family gene and one of HOXA2 gene and BCLXL gene in hematopoietic progenitor cells, CD34-positive cells, erythroid progenitor cells or Glycophorin A-positive pre-enucleation cells and amplifying the cells by culturing; and differentiating the amplified cells into the erythroid cells. 32 . The method of claim 31 , wherein the step of differentiating is performed by culturing the amplified cells after suppressing the forcible expression of MYC family gene and one of HOXA2 gene and BCLXL gene or removing MYC family gene and one of HOXA2 gene and BCLXL gene from the amplified cells. 33 . The method of claim 31 further comprising cryopreserving the amplified cells after the step of amplifying, wherein the step of differentiating is performed after the cryopreserved cells are thawed. 34 . A method for producing a blood product comprising: producing erythroid cells by the method of claim 31 ; and mixing the erythroid cells with other components to obtain the blood product. 35 . A cell population comprising hematopoietic progenitor cells, CD34-positive cells, erythroid progenitor cells or Glycophorin A-positive pre-enucleation cells which are introduced with MYC family gene and one of HOXA2 gene and BCLXL gene. 36 . A frozen cellular composition comprising the cell population of claim 35 . 37 . A kit for producing erythroid progenitor cells or erythroid cells comprising expression vectors for forced expression of MYC family gene and one of HOXA2 gene and BCLXL gene.
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- What does patent US2016115449A1 cover?
- The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. Th…
- Who is the assignee on this patent?
- Univ Tokyo
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0641. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Apr 28 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).