Activated platelet composition with tunable growth factor level

Patent metadata

Field	Value
Publication number	US-2016289664-A1
Application number	US-201514674971-A
Country	US
Kind code	A1
Filing date	Mar 31, 2015
Priority date	Mar 31, 2015
Publication date	Oct 6, 2016
Grant date	—

Abstract

Methods and systems for generating a tunable or customizable activated product composition are related. In certain embodiments, one or more of electric pulse parameters, flow rate, or sample container size are varied so as to generate the activated product composition. The activated product composition may be customized or optimized based for a particular patient or procedure.

First claim

1 . A method for releasing growth factors, comprising:
positioning a sample with respect to electrodes of a pulse generating apparatus;
specifying a set of electric pulse parameters, wherein different parameter values yield different levels of one or more growth factors in an activated product composition; and
exposing the sample to one or more electric pulses generated in accordance with the parameter values, wherein the sample, when exposed to the one or more electric pulses, yields the activated product composition having levels of the one or more growth factor determined at least in part by the set of electric pulse parameters.

2 . The method of claim 1 , wherein the sample comprises one of a platelet rich plasma sample, a platelet suspension, or a whole blood sample.

3 . The method of claim 1 , further comprising:
selecting a container or conduit size from a plurality of container sizes; and placing the sample within a container of the selected size prior, wherein positioning the sample comprises placing the container holding the sample within a sample holder between the electrodes, wherein the activated product composition has levels of the one or more factor determined at least in part by the container size.

4 . The method of claim 1 , wherein positioning the sample between the electrodes comprises flowing the sample through a conduit between the electrodes, wherein the activated product composition has levels of the one or more factor determined at least in part by one or both of the conduit diameter or the flow rate of the sample through the conduit.

5 . The method of claim 1 , wherein the set of electric pulse parameters comprise one or more of a voltage, an electric field, a current, a pulse width, an energy density, energy per platelet, or a number of pulses.

6 . The method of claim 1 , wherein the one or more factors comprise growth factors released by one or more of platelets, red blood cells, or white blood cells, including one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1.

7 . The method of claim 1 , wherein cells of the sample are not lysed in response to the one or more electric pulses.

8 . A method for releasing growth factors, comprising:
selecting a cuvette size, wherein different cuvette sizes yield different levels of one or more factors in an activated product composition; placing a sample within a cuvette of the selected size; placing the cuvette within a sample holder of a pulse generating apparatus; exposing the sample to one or more electric pulses, wherein the sample, when exposed to the one or more electric pulses, yields the activated product composition having levels of the one or more growth factor determined at least in part by the cuvette size.

9 . The method of claim 8 , further comprising:
specifying a set of electric pulse parameters, wherein different parameter values yield different levels of one or more growth factors in the activated product composition and wherein the activated product composition has levels of the one or more growth factor determined at least in part by the set of electric pulse parameters.

10 . The method of claim 9 , wherein the set of electric pulse parameters comprise one or more of a voltage, an electric field, a current, a pulse width, an energy density, or a number of pulses.

11 . The method of claim 8 , wherein the one or more growth factors comprise one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1.

12 . The method of claim 8 , wherein cells of the sample are not lysed in response to the one or more electric pulses.

13 . A method for customizing an activated blood-derived cell treatment, comprising:
determining a customized growth factor profile for treating a patient based on one or both of type of wound or a respective process of the wound healing cascade; selecting one or more electric pulse parameters and a cuvette size corresponding to the determined growth factor profile; exposing a sample placed in a cuvette of the selected size to one or more electric pulses generated based on the selected electric pulse parameters to generate an activated product composition having the growth factor profile.

14 . The method of claim 13 , wherein determining a growth factor profile comprises determining an absolute amount of growth factors released from one or more of platelets, red blood cells, or white blood cells, including one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1 to be present in the activated platelet composition.

15 . The method of claim 13 , wherein determining a growth factor profile comprises determining a relative amount of one or more of platelet derived growth factor, epidermal growth factor, vascular endothelial growth factor, or transforming growth factor beta 1 to be present in the activated product composition relative to another growth factor.

16 . The method of claim 13 , wherein selecting the one or more electric pulse parameters comprises specifying values for one or more of a voltage, an electric field, a current, a pulse width, an energy density, or a number of pulses.

17 . The method of claim 13 , wherein selecting the one or more electric pulse parameters comprises selecting a preconfigured pulse profile from a plurality of preconfigured pulse profiles.

18 . An electric pulse-generating system, comprising:
a sample holder configured to accept at least two different sizes of cuvettes;
pulse generating circuitry configured to generate one or more electrical pulses into a cuvette, when present in the sample holder;
one or more user input devices configured receive user inputs;
a non-transitory computer-readable memory storing one or more processor executable routines; and
a processor configured to access and execute the one or more processor executable routines stored in the computer-readable memory, wherein the processor executable routines, when executed cause acts to be performed comprising: receiving an input specifying one or more electric pulse parameters corresponding to a prescribed growth factor profile, wherein different electric pulse parameter values yield different levels of one or more growth factors in the growth factor profile; and generating one or more electric pulses based on the input using the pulse generating circuitry to generate an activated product composition having the prescribed growth factor profile.

19 . The system of claim 18 , wherein receiving the input comprises receiving a user selection of the growth factor profile from a plurality of growth factor profiles.

20 . The system of claim 18 , wherein the one or more electric pulse parameters comprise a voltage, an electric field, a current, a pulse width, an energy density, or a number of pulses.

Assignees

• Gen Electric

Inventors

• Neculaes Vasile Bogdan
• Becerra Juan Jose
• Morton Christine Angela
• Torres Andrew Soliz
• Caiafa Antonio

Classifications

• B01J2219/0803

  employing electric or magnetic energy · CPC title

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• B01J2219/0877

  Liquid · CPC title

• B01J19/087

  employing electric or magnetic energy · CPC title

• C12N2529/00

  Culture process characterised by the use of electromagnetic stimulation · CPC title