Lipid comprising polyunsaturated fatty acids

Patent metadata

Field	Value
Publication number	US-10899992-B2
Application number	US-201815975497-A
Country	US
Kind code	B2
Filing date	May 9, 2018
Priority date	Jun 15, 2012
Publication date	Jan 26, 2021
Grant date	Jan 26, 2021

Abstract

The present invention relates to extracted plant lipid, comprising fatty acids in an esterified form.

First claim

The invention claimed is:

1. Brassica napus seedoil comprising a total fatty acid content which comprises
i) a total monounsaturated fatty acid content which comprises oleic acid,
ii) a total saturated fatty acid content which comprises palmitic acid, or palmitic acid and myristic acid (C14:0),
iii) a total ω6 fatty acid content which comprises linoleic acid (LA) and γ-linolenic acid (GLA),
iv) a total ω3 fatty acid content which comprises α-linolenic acid (ALA), docosahexaenoic acid (DHA), stearidonic acid (SDA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and eicosatetraenoic acid (ETA),
wherein
a) palmitic acid is present at a level of between 2% and 16% of the total fatty acid content of the Brassica napus seedoil,
b) oleic acid is present at a level of between 1% and 60% of the total fatty acid content of the Brassica napus seedoil,
c) LA is present at a level of between 4% and 35% of the total fatty acid content of the Brassica napus seedoil,
d) GLA is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil,
e) ALA is present at a level of between 2% and 16% of the total fatty acid content of the Brassica napus seedoil,
f) the total saturated fatty acid content is between 4% and 25% of the total fatty acid content of the Brassica napus seedoil,
g) the ratio of the total ω6 fatty acid content to the total ω3 fatty acid content in the total fatty acid content of the Brassica napus seedoil is between 0.05 and 3.0,
h) myristic acid is present at a level of less than 2% of the total fatty acid content of the Brassica napus seedoil or is absent,
i) eicosatrienoic acid (ETrA) is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil or is absent, and
j) DHA is esterified in the form of triacylglycerols (TAG), wherein at least 70% of the DHA which is esterified in the form of TAG is esterified at the sn-1 or sn-3 position of the TAG,
wherein the level or content percentages correspond to a percentage by weight.

2. The Brassica napus seedoil of claim 1 , wherein the total saturated fatty acid content is between 6% and 20% of the total fatty acid content of the Brassica napus seedoil.

3. The Brassica napus seedoil of claim 1 , wherein the total fatty acid content of the Brassica napus seedoil comprises new ω3 fatty acids in the total ω3 fatty acid content and new ω6 fatty acids in the total ω6 fatty acid content, wherein the ratio of the new ω6 fatty acids to the new ω3 fatty acids in the Brassica napus seedoil is between 0.03 and 3.0.

4. The Brassica napus seedoil of claim 1 , wherein the Brassica napus seedoil comprises new ω3 fatty acids in the total ω3 fatty acid content, the new ω3 fatty acids comprising the SDA, ETA, EPA, DPA and DHA, and wherein the level of new ω3 fatty acids in the Brassica napus seedoil is between 10% and 20% of the total fatty acid content by weight.

5. Brassica napus seedoil comprising a total fatty acid content which comprises
i) a total monounsaturated fatty acid content which comprises oleic acid,
ii) a total saturated fatty acid content which comprises palmitic acid, or palmitic acid and myristic acid (C14:0),
iii) a total ω6 fatty acid content which comprises linoleic acid (LA) and γ-linolenic acid (GLA),
iv) a total ω3 fatty acid content which comprises α-linolenic acid (ALA), docosahexaenoic acid (DHA), stearidonic acid (SDA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and eicosatetraenoic acid (ETA),
wherein
a) palmitic acid is present at a level of between 2% and 16% of the total fatty acid content of the Brassica napus seedoil,
b) oleic acid is present at a level of between 1% and 60% of the total fatty acid content of the Brassica napus seedoil,
c) LA is present at a level of between 4% and 35% of the total fatty acid content of the Brassica napus seedoil,
d) GLA is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil,
e) the total saturated fatty acid content is between 4% and 25% of the total fatty acid content of the Brassica napus seedoil,
f) the ratio of the total ω6 fatty acid content to the total ω3 fatty acid content in the total fatty acid content of the Brassica napus seedoil is between 0.05 and 3.0,
g) myristic acid is present at a level of less than 2% of the total fatty acid content of the Brassica napus seedoil or is absent,
h) eicosatrienoic acid (ETrA) is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil or is absent, and
i) DHA is esterified in the form of triacylglycerols (TAG), wherein at least 70% of the DHA which is esterified in the form of TAG is esterified at the sn-1 or sn-3 position of the TAG,
wherein the level or content percentages correspond to a percentage by weight.

6. The Brassica napus seedoil of claim 5 , wherein the Brassica napus seedoil comprises new ω3 fatty acids in the total ω3 fatty acid content, the new ω3 fatty acids comprising the SDA, ETA, EPA, DPA and DHA, and wherein the level of new ω3 fatty acids in the Brassica napus seedoil is between 10% and 20% of the total fatty acid content by weight.

7. Brassica napus seedoil comprising a total fatty acid content which comprises
i) a total monounsaturated fatty acid content which comprises oleic acid,
ii) a total saturated fatty acid content which comprises palmitic acid, or palmitic acid and myristic acid (C14:0),
iii) a total ω6 fatty acid content which comprises linoleic acid (LA) and γ-linolenic acid (GLA),
iv) a total ω3 fatty acid content which comprises α-linolenic acid (ALA), docosahexaenoic acid (DHA), stearidonic acid (SDA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and eicosatetraenoic acid (ETA),
wherein
a) palmitic acid is present at a level of between 2% and 16% of the total fatty acid content of the Brassica napus seedoil,
b) oleic acid is present at a level of 27-33% of the total fatty acid content of the Brassica napus seedoil,
c) LA is present at a level of between 4% and 35% of the total fatty acid content of the Brassica napus seedoil,
d) GLA is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil,
e) the total saturated fatty acid content is between 4% and 25% of the total fatty acid content of the Brassica napus seedoil,
f) myristic acid is present at a level of less than 2% of the total fatty acid content of the Brassica napus seedoil or is absent,
g) eicosatrienoic acid (ETrA) is present at a level of less than 4% of the total fatty acid content of the Brassica napus seedoil or is absent, and
h) DHA is esterified in the form of triacylglycerols (TAG), wherein at least 70% of the DHA which is esterified in the form of TAG is esterified at the sn-1 or sn-3 position of the TAG,

wherein the level or content percentages correspond to a percentage by weight.

8. The Brassica napus seedoil of claim 7 , wherein the Brassica napus seedoil comprises new ω3 fatty acids in the total ω3 fatty acid content, the new ω3 fatty acids comprising the SDA, ETA, EPA, DPA and DHA, and wherein the level of new ω3 fatty acids in the Brassica napus seedoil is between 10% and 20% of the total fatty acid content by weight.

9. The Brassica napus seedoil of claim 1 , wherein at least 80% of the DHA esterified in the form of TAG in the total fatty acid content of the Brassica napus seedoil is at the sn-1 or sn-3 position of the TAG.

10. The Brassica napus seedoil of claim 4 , wherein at least 80% of the DHA esterified in the form of TAG in the total fatty acid content of the Brassica napus seedoil

Assignees

• Commw Scient Ind Res Org
• Grains Res & Dev Corp
• Nuseed Pty Ltd

Inventors

• Petrie James Robertson
• Singh Surinder Pal
• De Feyter Robert Charles

Classifications

• A01H6/20

  Brassicaceae, e.g. canola, broccoli or rucola · CPC title

• A01H1/104

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• A01H5/10

  Seeds · CPC title

• C07C57/03

  Monocarboxylic acids · CPC title

• C07C57/02

  with only carbon-to-carbon double bonds as unsaturation · CPC title