Fatty acid elongase and uses thereof

Patent metadata

Field	Value
Publication number	US-9347049-B2
Application number	US-201013511386-A
Country	US
Kind code	B2
Filing date	Nov 23, 2010
Priority date	Nov 24, 2009
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

The present invention relates to nucleic acids derived from Sphaeroforma arctica . The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.

First claim

The invention claimed is:

1. An isolated polynucleotide comprising an expression control sequence operatively linked to a heterologous nucleic acid sequence selected from the group consisting of:
a) the nucleic acid sequence of SEQ ID NO: 3;
b) a nucleic acid sequence encoding a polypeptide having the amino acid sequence of SEQ ID NO: 4;
c) a nucleic acid sequence having at least 95% sequence identity to the nucleic acid sequence of SEQ ID NO: 3, wherein said nucleic acid sequence encodes a polypeptide having A6-elongase activity;
d) a nucleic acid sequence encoding a polypeptide having A6-elongase activity and having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 4.

2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises a terminator sequence operatively linked to the nucleic acid sequence.

3. A vector comprising the polynucleotide of claim 1 .

4. A transgenic host cell comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide.

5. A non-human transgenic organism comprising:
a) the polynucleotide of claim 1 ; or
b) a vector comprising said polynucleotide,
wherein the non-human transgenic organism is a plant, plant part, plant seed, or microorganism.

6. The non-human transgenic organism of claim 5 , wherein the microorganism is a fungus, algae, moss, or yeast.

7. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell; and
b) obtaining said polyunsaturated fatty acids from said host cell.

8. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the non-human transgenic organism of claim 5 under conditions which allow for the production of polyunsaturated fatty acids in said non-human transgenic organism; and
b) obtaining said polyunsaturated fatty acids from said non-human transgenic organism.

9. The method of claim 8 , wherein the polyunsaturated fatty acid is arachidonic acid (ARA), eicosapentaenoic acid (EPA), and/or docosahexaenoic acid (DHA).

10. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising:
a) providing a polyunsaturated fatty acid produced by the method of claim 8 ; and
b) formulating said polyunsaturated fatty acid as an oil-, lipid- or fatty acid-composition.

11. The method of claim 10 , wherein the oil-, lipid- or fatty acid-composition is used for feed, foodstuffs, cosmetics, or pharmaceuticals.

12. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining said polyunsaturated fatty acids from said plant or seeds thereof.

13. The method of claim 12 , wherein the polyunsaturated fatty acids are obtained from the seeds of said plant.

14. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising:
a) providing a polyunsaturated fatty acid produced by the method of claim 12 ; and
b) formulating said polyunsaturated fatty acid as an oil-, lipid- or fatty acid-composition.

15. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining an oil-, lipid- or fatty acid-composition from said plant or seeds thereof.

16. The method of claim 15 , wherein the oil-, lipid- or fatty acid-composition is obtained from the seeds of said plant.

17. The polynucleotide of claim 1 , wherein said heterologous nucleic acid sequence encodes a polypeptide having Δ6-elongase activity and having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 4.

18. The transgenic host cell of claim 4 , wherein the transgenic host cell is a plant cell, or a microorganism cell.

19. The transgenic host cell of claim 4 , wherein the transgenic host cell is a yeast, fungus, algae, moss, or an insect cell.

20. The method of claim 15 further comprising obtaining polyunsaturated fatty acids from said oil-, lipid- or fatty acid-composition.

Assignees

• Bauer Jörg
• Qiu Xiao
• Vrinten Patricia
• Basf Plant Science Co Gmbh

Inventors

• Bauer Jörg
• Qiu Xiao
• Vrinten Patricia

Classifications

• C12N9/0083

  Miscellaneous (1.14.99) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N9/1029 Primary

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12P7/6427

  Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone · CPC title