Lipid comprising polyunsaturated fatty acids
US-2015045569-A1 · Feb 12, 2015 · US
Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9453183B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9453183-B2 |
| Application number | US-201414323512-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 3, 2014 |
| Priority date | Apr 22, 2004 |
| Publication date | Sep 27, 2016 |
| Grant date | Sep 27, 2016 |
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Abstract
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The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
First claim
Opening claim text (preview).
The invention claimed is: 1. A cell comprising eicosapentaenoic acid in an esterified form as part of triacylglycerols in the cell and an exogenous polypeptide having Δ5 desaturase activity whose amino acid sequence is at least 90% identical to the amino acid sequence set forth as SEQ ID NO: 60. 2. The cell of claim 1 , wherein at least 50% of the eicosapentaenoic acid in the cell is incorporated into triacylglycerols. 3. The cell of claim 1 , further comprising docosahexaenoic acid in an esterified form as part of triacylglycerols in the cell, wherein at least 50% of the docosahexaenoic acid in the cell is incorporated into triacylglycerols. 4. The cell of claim 1 , wherein the total fatty acid content in the cell comprises 2.5% (w/w) C20 ω3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid. 5. The cell of claim 4 , wherein the total fatty acid content in the cell comprises 0.13% (w/w) docosapentaenoic acid. 6. The cell of claim 5 , wherein the total fatty acid content in the cell comprises 0.5% (w/w) docosapentaenoic acid. 7. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is identical to the amino acid sequence set forth as SEQ ID NO: 60. 8. A process of producing oil comprising i) obtaining the cell of claim 1 , and ii) extracting oil from the cell. 9. The process of claim 8 , wherein the cell is a plant cell. 10. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is at least 95% identical to the amino acid sequence set forth as SEQ ID NO: 60. 11. The cell of claim 1 , wherein the amino acid sequence of the exogenous polypeptide is at least 99% identical to the amino acid sequence set forth as SEQ ID NO: 60.
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Classifications
Nutrients, e.g. vitamins, minerals · CPC title
Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00) · CPC title
Genes encoding for enzymes or proenzymes · CPC title
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title
Fatty acids; Fats; Products containing oils or fats · CPC title
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Frequently asked questions
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- What does patent US9453183B2 cover?
- The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzyme…
- Who is the assignee on this patent?
- Singh Surinder Pal, Robert Stanley Suresh, Nichols Peter David, and 5 more
- What technology area does this patent fall under?
- Primary CPC classification C11C3/12. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 27 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).