Fatty acid desaturase and uses thereof

Patent metadata

Field	Value
Publication number	US-9388436-B2
Application number	US-201013511069-A
Country	US
Kind code	B2
Filing date	Nov 23, 2010
Priority date	Nov 24, 2009
Publication date	Jul 12, 2016
Grant date	Jul 12, 2016

Abstract

The present invention relates to nucleic acids derived from Limonomyces roseipellis . The invention also relates to the individual coding sequences and to proteins encoded by these sequences as well to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.

First claim

The invention claimed is:

1. A polynucleotide comprising an expression control sequence operatively linked and heterologous to a nucleic acid sequence selected from the group consisting of:
a) the nucleic acid sequence of SEQ ID NO: 1;
b) a nucleic acid sequence encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2;
c) a nucleic acid sequence being at least 85% identical to the nucleic acid sequence of a), wherein said nucleic acid sequence encodes a polypeptide having 415-desaturase activity; and
d) a nucleic acid sequence encoding a polypeptide having Δ15-desaturase activity and having an amino acid sequence which is at least 85% identical to the amino acid sequence of SEQ ID NO: 2.

2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises a terminator sequence operatively linked to the nucleic acid sequence.

3. A vector comprising the polynucleotide of claim 1 , wherein said polynucleotide optionally further comprises a terminator sequence operatively linked to the nucleic acid sequence.

4. A host cell comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide, wherein said polynucleotide optionally further comprises a terminator sequence operatively linked to the nucleic acid sequence.

5. A method for the manufacture of a polypeptide, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of a polypeptide encoded by the polynucleotide; and
b) obtaining said polypeptide from the host cell of step a).

6. A non-human transgenic organism comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide.

7. The non-human transgenic organism of claim 6 , wherein the organism is a plant, a plant part, a plant seed, a fish or a microorganism.

8. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell; and
b) obtaining said polyunsaturated fatty acids from said host cell.

9. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating the non-human transgenic organism of claim 6 under conditions which allow for the production of polyunsaturated fatty acids in said non-human transgenic organism; and
b) obtaining said polyunsaturated fatty acids from said non-human transgenic organism.

10. The method of claim 9 , wherein the polyunsaturated fatty acid is arachidonic acid (ARA), eicosapentaenoic acid (EPA), and/or docosahexaenoic acid (DHA).

11. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising obtaining polyunsaturated fatty acids according to the method of claim 9 , and formulating the polyunsaturated fatty acids in an oil-, lipid- or fatty acid-composition.

12. The method of claim 11 , wherein the oil-, lipid- or fatty acid-composition is to be used for feed, foodstuffs, cosmetics or pharmaceuticals.

13. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising obtaining polyunsaturated fatty acids according to the method of claim 10 , and formulating the polyunsaturated fatty acids in an oil-, lipid- or fatty acid-composition.

14. The polynucleotide of claim 1 , wherein said nucleic acid sequence encodes a polypeptide having Δ15-desaturase activity and having a conversion rate more than 50%.

15. The polynucleotide of claim 1 , wherein the nucleic acid sequence encodes a polypeptide having Δ15-desaturase activity and having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.

16. The polynucleotide of claim 1 , wherein the nucleic acid sequence encodes a polypeptide having Δ15-desaturase activity and having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.

17. A method for the manufacture of polyunsaturated fatty acids, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining said polyunsaturated fatty acids from said plant or seeds thereof.

18. The method of claim 17 , comprising obtaining an oil-, lipid- or fatty acid-composition from said plant or seeds thereof, and obtaining the polyunsaturated fatty acids from said oil-, lipid- or fatty acid-composition.

19. A method for the manufacture of an oil-, lipid- or fatty acid-composition, comprising:
a) cultivating a plant comprising the polynucleotide of claim 1 or a vector comprising said polynucleotide under conditions which allow for the production of polyunsaturated fatty acids in said plant or seeds thereof; and
b) obtaining an oil-, lipid- or fatty acid-composition from said plant or seeds thereof.

20. The method of claim 17 , wherein the polyunsaturated fatty acids are obtained from the seeds of said plant.

Assignees

• Bauer Jörg
• Qiu Xiao
• Vrinten Patricia
• Basf Plant Science Co Gmbh

Inventors

• Bauer Jörg
• Qiu Xiao
• Vrinten Patricia

Classifications

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12P7/6427 Primary

  Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone · CPC title

• C12P7/6434

  Docosahexenoic acids [DHA] · CPC title