Chromatographic materials

Patent metadata

Field	Value
Publication number	US-9925521-B2
Application number	US-201314401385-A
Country	US
Kind code	B2
Filing date	May 15, 2013
Priority date	May 15, 2012
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W) a (Q) b (T) c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

First claim

The invention claimed is:

1. A method for mitigating or preventing retention drift in supercritical fluid chromatography, sub-critical fluid chromatography, or carbon dioxide based chromatography comprising:
chromatographically separating a sample using a chromatographic device comprising a chromatographic stationary phase represented by Formula 1:
[X](W) a (Q) b (T) c   Formula 1

wherein:
X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof;
W is an hydroxyl group on the surface of X;
Q is represented by:

T is represented by one of:

wherein each Z group independently comprises:
a) a surface attachment group having the formula (B 1 ) x (R 5 ) y (R 6 ) z Si—
wherein x is an integer from 1-3,
y is an integer from 0-2,
z is an integer from 0-2,
and x+y+z=3
each occurrence of R 5 and R 6 independently represents methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, or a zwitterion group; and
B 1 represents a siloxane bond;

b) an attachment to a surface organofunctional hybrid group selected from the group consisting of a direct carbon-carbon bond, a heteroatom linkage, ester linkage, ether linkage, thioether linkage, amine linkage, amide linkage, imide linkage, urea linkage, carbonate linkage, carbamate linkage, heterocycle linkage, triazole linkage and urethane linkage; or
c) an adsorbed, surface group that is not covalently attached to the surface of X; and b and c are positive numbers, 0.05≤(b/c)≤100, and a≥0,
thereby mitigating or preventing retention drift.

2. The method of claim 1 , wherein T is represented by:

Assignees

• Waters Technologies Corp

Inventors

• Wyndham Kevin D
• Morris Michael F
• Brousmiche Darryl W
• Hill Jason F
• Fairchild Jacob N

Classifications

• B01D15/26 Primary

  characterised by the separation mechanism · CPC title

• B01J20/28064

  being in the range 500-1000 m2/g · CPC title

• B01J20/284

  based on alumina · CPC title

• B01J20/28061

  being in the range 100-500 m2/g · CPC title

• B01J20/3246

  having a well defined chemical structure · CPC title