OMPA and ASP14 in vaccine compositions and as diagnostic targets

Patent metadata

Field	Value
Publication number	US-9248174-B2
Application number	US-201314408760-A
Country	US
Kind code	B2
Filing date	Jun 24, 2013
Priority date	Jun 27, 2012
Publication date	Feb 2, 2016
Grant date	Feb 2, 2016

Abstract

Anaplasma phagocytophilum surface proteins Asp14 and OmpA and homologous genes from Anaplasmatacaea family members are used in compositions suitable for vaccines to treat or prevent infections caused by tick-born bacteria of the Anaplasmatacaea family. Asp14 and/or OmpA proteins or peptide fragments may be used in combination with other Anaplasmatacaea surface proteins to elicit an immune response. Furthermore, antibodies to Asp14 and/or OmpA proteins can be used in diagnostic methods to determine whether an individual has contracted an Anaplasmatacaea infection. Because of the conserved invasin domains in the surface proteins, a wide range of Anaplasmatacaea infections may be diagnosed, treated or prevented using compositions of the invention.

First claim

What is claimed is:

1. A method of determining if a subject has been exposed to or is infected with an obligate intracellular Anaplasmataceae bacterium selected from the group consisting of Anaplasma phagocytophilum, Anaplasma marginale , and Anaplasma platys , wherein said subject is suspected of having a zoonotic disease caused by an obligate intracellular Anaplasmataceae bacterium, comprising the steps of
contacting a test sample from said subject, under conditions that allow polypeptide-antibody complexes to form, with a composition that includes one or more polypeptides, at least one of which comprises an amino acid sequence as set forth in SEQ ID NO:1 or SEQ ID NO:2,
detecting one or more polypeptide-antibody complexes in said test sample, wherein the detection is an indication that antibodies specific for Anaplasmataceae Asp 14 are present in the test sample, and
determining said subject has been exposed to or is infected with said Anaplasmataceae bacterium if said antibodies specific for Anaplasmataceae Asp 14 are present in the test sample.

2. The method of claim 1 , wherein said contacting and detecting steps are performed using an assay selected from the group consisting of an immunoblot and an enzyme-linked immunosorbent assay (ELISA).

3. The method of claim 1 , wherein said subject is a human, and said zoonotic disease is human granulocytic anaplasmosis (HGA).

4. The method of claim 1 , wherein said subject is an animal and said zoonotic disease is anaplasmosis.

5. The method of claim 1 , wherein said one or more polypeptides includes a polypeptide comprising an amino acid sequence as set forth in SEQ ID NO:2.

6. A method of determining if a subject has been exposed to or is infected with Anaplasma phagocytophilum , wherein said subject is suspected of having a zoonotic disease caused by Anaplasma phagocytophilum , comprising the steps of
contacting a test sample from said subject, under conditions that allow polypeptide-antibody complexes to form, with a composition that includes one or more polypeptides, at least one of which comprises an amino acid sequence as set forth in SEQ ID NO:3,
detecting one or more polypeptide-antibody complexes in said test sample, wherein the detection is an indication that antibodies specific for Anaplasma phagocytophilum Asp14 are present in the test sample, and
determining said subject has been exposed to or is infected with said Anaplasma phagocytophilum if said antibodies specific for Anaplasma phagocytophilum Asp14 are present in the test sample.

7. The method of claim 1 , wherein said test sample is a body fluid selected from the group consisting of blood, plasma, serum, urine, and saliva.

Assignees

• Univ Virginia Commonwealth

Inventors

• Carlyon Jason A

Classifications

• A61P33/02

  Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title

• A61K39/0233 Primary

  Rickettsiales, e.g. Anaplasma · CPC title

• A61K2039/6081

  Albumin; Keyhole limpet haemocyanin [KLH] · CPC title

• G01N2333/29

  from Richettsiales (o) · CPC title