Signal sequence that induces protein secretion in intestinal microbiome
US-2024190925-A1 · Jun 13, 2024 · US
US9605033B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9605033-B2 |
| Application number | US-201514729758-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 3, 2015 |
| Priority date | Aug 31, 2006 |
| Publication date | Mar 28, 2017 |
| Grant date | Mar 28, 2017 |
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The present invention concerns gp19 immunoreactive compositions for E. canis and compositions related thereto, including vaccines, antibodies, polypeptides, peptides, and polynucleotides. In particular, epitopes for E. canis gp19 are disclosed.
Opening claim text (preview).
What is claimed is: 1. A method of detecting an anti- E. canis antibody in an individual, comprising the step of assaying a biological sample from the individual for the presence of an antibody that immunologically binds to a polypeptide comprising the amino acid sequence of SEQ ID NO: 13 or an amino acid sequence that is 95% identical to SEQ ID NO: 13, wherein the polypeptide is 24 to 75 amino acids in length and the assaying comprises contacting the sample with the polypeptide. 2. The method of claim 1 , wherein the sample is assayed by contacting with the polypeptide comprising the amino acid sequence of SEQ ID NO: 13. 3. The method of claim 2 , wherein the polypeptide is bound to a solid support. 4. The method of claim 3 , wherein the solid support is a column matrix, a well of a microtiter plate, a dot blot membrane, or a Western blot membrane. 5. The method of claim 1 , wherein the sample is assayed by contacting with the polypeptide comprising the amino acid sequence that is at least 95% identical to SEQ ID NO: 13.
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