Ketol-acid reductoisomerase using nadh
US-2015111269-A1 · Apr 23, 2015 · US
Ketol-acid reductoisomerase using NADH
US9238801B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9238801-B2 |
| Application number | US-201313838869-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 15, 2013 |
| Priority date | Dec 20, 2007 |
| Publication date | Jan 19, 2016 |
| Grant date | Jan 19, 2016 |
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Abstract
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Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
First claim
Opening claim text (preview).
What is claimed is: 1. A recombinant mutant ketol-acid reductoisomerase enzyme having the amino acid sequence of SEQ ID NO: 29. 2. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 24 wherein the substitution is an amino acid substitution of phenylalanine. 3. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 50 wherein the substitution is an amino acid substitution of alanine. 4. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 52 wherein the substitution is an amino acid substitution of aspartic acid. 5. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 53 wherein the substitution is an amino acid substitution of alanine. 6. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 61 wherein the substitution is an amino acid substitution of phenylalanine. 7. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme further comprises a substitution at position 115 wherein the substitution is an amino acid substitution of leucine. 8. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme further comprises a substitution at position 165 wherein the substitution is an amino acid substitution of methionine. 9. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 33 wherein the substitution is an amino acid substitution of leucine. 10. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 80 wherein the substitution is an amino acid substitution of isoleucine. 11. The recombinant mutant ketol-acid reductoisomerase enzyme of claim 1 , wherein the enzyme comprises a substitution at position 156 wherein the substitution is an amino acid substitution of valine.
Assignees
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Classifications
for yeasts · CPC title
- C12N9/0006Primary
acting on CH-OH groups as donors (1.1) · CPC title
Butanols · CPC title
involving oxidoreductase · CPC title
Hydroxy-carboxylic acids · CPC title
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- What does patent US9238801B2 cover?
- Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
- Who is the assignee on this patent?
- Butamax Tm Advanced Biofuels, Butamax Advanced Biofuels Llc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0006. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 19 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 11 related publications on this page (citations in our corpus or others sharing the same primary CPC).