Production of isobutanol by a microorganism with enhanced acetolactate synthase activity

US8945859B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8945859-B2
Application numberUS-201313839246-A
CountryUS
Kind codeB2
Filing dateMar 15, 2013
Priority dateOct 26, 2005
Publication dateFeb 3, 2015
Grant dateFeb 3, 2015

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Abstract

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Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

First claim

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What is claimed is: 1. A method comprising: (a) determining the acetolactate synthase (ALS) activity of a cell free extract prepared from a host cell expressing a heterologous ALS enzyme; and (b) expressing a heterologous DNA molecule encoding the ALS enzyme in a recombinant microorganism expressing an engineered isobutanol biosynthetic pathway, wherein the cell free extract comprises higher ALS activity than a control cell free extract prepared from a host cell not expressing…

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What does patent US8945859B2 cover?
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Who is the assignee on this patent?
Butamax ™ Advanced Biofuels Llc, Butamax Advanced Biofuels Llc
What technology area does this patent fall under?
Primary CPC classification C12P7/16. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).