Making c4+ products in bacteria
US-2015376658-A1 · Dec 31, 2015 · US
US8945859B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945859-B2 |
| Application number | US-201313839246-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 15, 2013 |
| Priority date | Oct 26, 2005 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
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What is claimed is: 1. A method comprising: (a) determining the acetolactate synthase (ALS) activity of a cell free extract prepared from a host cell expressing a heterologous ALS enzyme; and (b) expressing a heterologous DNA molecule encoding the ALS enzyme in a recombinant microorganism expressing an engineered isobutanol biosynthetic pathway, wherein the cell free extract comprises higher ALS activity than a control cell free extract prepared from a host cell not expressing…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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