Chromatographic materials

Patent metadata

Field	Value
Publication number	US-2019217273-A1
Application number	US-201916359328-A
Country	US
Kind code	A1
Filing date	Mar 20, 2019
Priority date	May 15, 2012
Publication date	Jul 18, 2019
Grant date	—

Abstract

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W) a (Q) b (T) c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

First claim

1 . A chromatographic stationary phase material for normal phase chromatography, high-pressure liquid chromatography, solvated gas chromatography, supercritical fluid chromatography, sub-critical fluid chromatography, carbon dioxide based chromatography, hydrophilic interaction liquid chromatography or hydrophobic interaction liquid chromatography represented by Formula I:
[X](W) a (Q) b (T) c   Formula I
wherein: X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof; W is an hydroxyl group on the surface of X; Q is represented by:

T is represented by:

wherein Z is a chemically reactive group; and
b and c are positive numbers, 0.05≤(b/c)≤100, and a≥0.

2 - 62 . (canceled)

63 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a plurality of particles.

64 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a monolith.

65 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a superficially porous material.

66 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has chromatographically enhancing pore geometry.

67 . The chromatographic stationary phase material of claim 63 , wherein the plurality of particles have sizes between about 1.5 and 5 microns.

68 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a surface area of about 25 to 1100 m 2 /g.

69 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a pore volume of about 0.2 to 2.0 cm 3 /g.

70 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a micropore surface area of less than about 105 m 2 /g.

71 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has an average pore diameter of about 20 to 1500 Å.

72 . The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥0.5 μmol/m 2 .

73 . The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥1.5 μmol/m 2 .

74 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of an inorganic material with a hybrid surrounding layer.

75 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with an inorganic surface layer.

76 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with a different hybrid surface layer.

77 . A method for mitigating or preventing retention drift in normal phase chromatography, high-pressure liquid chromatography, solvated gas chromatography, supercritical fluid chromatography, sub-critical fluid chromatography, carbon dioxide based chromatography, hydrophilic interaction liquid chromatography or hydrophobic interaction liquid chromatography comprising:
chromatographically separating a sample using a chromatographic device comprising a chromatographic stationary phase represented by Formula I:
[X](W) a (Q) b (T) c   Formula I

wherein: X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof; W is an hydroxyl group on the surface of X; Q is represented by:

T is represented by:

wherein Z group is a chemically reactive group; and
b and c are positive numbers, 0.05≤(b/c)≤100, and a≥0,
thereby mitigating or preventing retention drift.

Assignees

• Waters Technologies Corp

Inventors

• Wyndham Kevin D
• Morris Michael F
• Brousmiche Darryl W
• Hill Jason F
• Fairchild Jacob N

Classifications

• B01J20/22

  comprising organic material · CPC title

• B01D15/305

  Hydrophilic interaction chromatography [HILIC] · CPC title

• B01D15/26 Primary

  characterised by the separation mechanism · CPC title

• B01J20/28073

  being in the range 0.5-1.0 ml/g · CPC title

• B01J20/28059

  being less than 100 m2/g · CPC title