Magnetic removal or identification of damaged or compromised cells or cellular structures

Patent metadata

Field	Value
Publication number	US-12332239-B2
Application number	US-202318139242-A
Country	US
Kind code	B2
Filing date	Apr 25, 2023
Priority date	Aug 29, 2012
Publication date	Jun 17, 2025
Grant date	Jun 17, 2025

Abstract

A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

First claim

The invention claimed is:

1. A method for processing, the method comprising:
a) contacting sperm cells with a plurality of magnetic particles, the plurality of magnetic particles having an average hydrodynamic diameter of 300 nm and each magnetic particle in the plurality of magnetic particles comprising a negative zeta potential charge and a chargeable silicon-containing compound, the magnetic particles binding to the sperm cells through an electrical charge interaction; and
b) applying a magnetic field to the plurality of magnetic particles.

2. The method of claim 1 , wherein each magnetic particle comprises Fe 3 O 4 .

3. The method of claim 1 , wherein the chargeable silicon-containing compound comprises 2-(carbomethoxy)ethyltrimethoxysilane.

4. The method of claim 1 , further comprising the step of diluting the sperm cells with a buffered media prior to step a).

5. The method of claim 1 , wherein each magnetic particle in the plurality of magnetic particles is between 30 nm to 1000 nm in size.

Assignees

• Inguran Llc

Inventors

• Krug Kristie

Classifications

• G01N33/56966

  Animal cells · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• G01N33/54333

  Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title