Magnetic removal or identification of damaged or compromised cells or cellular structures

Patent metadata

Field	Value
Publication number	US-10324086-B2
Application number	US-201715713391-A
Country	US
Kind code	B2
Filing date	Sep 22, 2017
Priority date	Aug 29, 2012
Publication date	Jun 18, 2019
Grant date	Jun 18, 2019

Abstract

A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

First claim

The invention claimed is:

1. A method of separating viable sperm from dead or damaged sperm comprising:
diluting a semen sample comprising viable sperm, and dead or damaged sperm, with a buffered media;
adding magnetic particles to the diluted semen sample to form an admixture, wherein each magnetic particle is no greater than 1000 nm and coated with a chargeable silicon-containing compound and a chargeable silicon-containing compound;
binding a portion of the dead or damaged sperm to the magnetic particles in the diluted admixture through an electrical charge interaction; and
magnetically separating the bound dead or damaged sperm from unbound viable sperm.

2. The method of claim 1 , wherein the magnetic particles comprise Fe 3 O 4 .

3. The method of claim 1 , wherein the magnetic particles comprise 2-(carbomethoxy)ethyltrimethoxysilane.

4. The method of claim 1 , wherein each magnetic particle is between 30 nm to 1000 nm in size.

5. The method of claim 1 , further comprising the step of centrifuging the semen sample prior to the step of adding the magnetic particles.

6. The method of claim 5 , further comprising the step of decanting the centrifuged semen sample.

7. The method of claim 6 , further comprising the step of resuspending the centrifuged semen sample in buffered media.

Assignees

• Inguran Llc

Inventors

• Krug Kristie

Classifications

• G01N33/5434 Primary

  using magnetic particle immunoreagent carriers which constitute new materials per se · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C1/015

  by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation · CPC title

• G01N33/56966

  Animal cells · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title