Cell processing using magnetic particles

Patent metadata

Field	Value
Publication number	US-11630108-B2
Application number	US-201916537402-A
Country	US
Kind code	B2
Filing date	Aug 9, 2019
Priority date	Aug 29, 2012
Publication date	Apr 18, 2023
Grant date	Apr 18, 2023

Abstract

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.

First claim

The invention claimed is:

1. A method of processing sperm cells comprising the steps of:
a) forming a stream comprising the sperm cells;
b) determining whether a property of interest is present or absent in the sperm cells in the stream;
c) ablating or photodamaging a subpopulation of the sperm cells based on the presence or absence of the property of interest in the sperm cells;
d) collecting the sperm cells;
e) adding magnetic particles to the collected sperm cells to form a mixture, the magnetic particles having a diameter of 30 to 1000 nm and comprising a chargeable silicon-containing compound, the magnetic particles binding to the ablated or photodamaged subpopulation of sperm cells solely through an electrical charge interaction;
f) applying a magnetic field to the magnetic particle-bound sperm cells; and
g) removing the magnetic particle-bound subpopulation from the mixture.

2. The method of claim 1 , the magnetic particles comprising an outer layer comprising a polysaccharide, an alkylsilane, a polylactic acid, a polycaprolactone, polyhydroxybutyrate-valerate or a polyolefin.

3. The method of claim 2 , the outer layer further comprising a chargeable, or charged, moiety.

4. The method of claim 3 , the chargeable, or charged, moiety comprising a metal, an oxide, a carboxylate, an amine, an amide, a carbamide, a sulfate, a sulfonate, a sulfite, a phosphonate, a phosphate, a halide or a hydroxide.

5. The method of claim 1 , wherein the mixture has a pH of 6.5 to 8.0.

6. The method of claim 1 , wherein the mixture comprises a buffer.

7. The method of claim 1 , wherein the mixture comprises sperm cells at a concentration of 120×10 6 cells/ml to 500×10 6 cells/ml.

8. The method of claim 1 , wherein the magnetic particles are non-toxic to the sperm cells.

Assignees

• Inguran Llc

Inventors

• Krug Kristie

Classifications

• G01N33/54346

  Nanoparticles · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C1/01 Primary

  by addition of magnetic adjuvants · CPC title

• G01N15/10

  Investigating individual particles · CPC title

• G01N33/56966 Primary

  Animal cells · CPC title