Magnetic removal or identification of damaged or compromised cells or cellular structures

Patent metadata

Field	Value
Publication number	US-9804153-B2
Application number	US-201313974139-A
Country	US
Kind code	B2
Filing date	Aug 23, 2013
Priority date	Aug 29, 2012
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

First claim

The invention claimed is:

1. A method for magnetic cellular separation, the method comprising:
contacting a composition with a biological sample to form a mixture, the composition comprising particles, wherein each particle is no greater than 1000 nm and is comprised of: i) a magnetic substrate, the magnetic substrate characterized by a magnetic susceptibility greater than zero; and ii) a chargeable silicon-containing compound, the chargeable silicon-containing compound coating at least a portion of the magnetic substrate; and the biological sample comprising viable sperm cells and damaged or dead sperm cells, the particles binding to the damaged or dead sperm cells solely through an electrical charge interaction;
applying a magnetic field to the mixture; and
separating the damaged or dead sperm cells from the viable sperm cells.

2. The method of claim 1 , the particles comprising a negative zeta potential charge, and wherein each particle is between 30 nm to 1000 nm in size.

3. The method of claim 1 , further comprising the step of subjecting the biological sample to sperm cell detection.

4. The method of claim 3 , the sperm cell detection comprising fluorescence detection.

Assignees

• Inguran Llc

Inventors

• Krug Kristie Marie

Classifications

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• G01N33/5434 Primary

  using magnetic particle immunoreagent carriers which constitute new materials per se · CPC title

• B03C1/02

  acting directly on the substance being separated · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title