Transgenic corn event MON95275 and methods for detection and uses thereof

Patent metadata

Field	Value
Publication number	US-12188034-B2
Application number	US-202217829963-A
Country	US
Kind code	B2
Filing date	Jun 1, 2022
Priority date	Apr 24, 2020
Publication date	Jan 7, 2025
Grant date	Jan 7, 2025

Abstract

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

First claim

What is claimed is:

1. A pair of DNA molecules, comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule, that function as DNA primers when used together in an amplification reaction with a sample containing corn event MON95275 template DNA to produce an amplicon diagnostic for the presence of said corn event MON95275 DNA in said sample,
wherein said first DNA molecule comprises at least 15 contiguous nucleotides of SEQ ID NO:9 or a complement thereof,
wherein said second DNA molecule comprises at least 15 contiguous nucleotides of SEQ ID NO:11, SEQ ID NO: 12, or a complement thereof,
wherein said amplicon comprises the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO: 7, and SEQ ID NO:8.

2. A method of detecting the presence of a DNA segment diagnostic for corn event MON95275 DNA in a sample, said method comprising:
a) contacting said sample with the pair of DNA molecules of claim 1 ;
b) performing an amplification reaction sufficient to produce a DNA amplicon; and
c) detecting the presence of said DNA amplicon in said reaction,
wherein said DNA amplicon comprises the nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO: 6, SEQ ID NO:7, and SEQ ID NO:8.

3. The method of claim 2 , wherein the DNA amplicon comprises the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO:2.

4. The method of claim 2 , wherein the DNA amplicon comprises the nucleotide sequence of SEQ ID NO:3 or SEQ ID NO:4.

5. The method of claim 2 , wherein the DNA amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO: 7, and SEQ ID NO:8.

Assignees

• Monsanto Technology Llc

Inventors

• Brown Sarah L
• Flasinski Stanislaw
• Pan Aihong
• Stelzer Jason W
• Windler Heidi M
• Yin Yong

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/158

  Expression markers · CPC title

• A01H6/4684

  Zea mays [maize] · CPC title

• C07K14/415

  from plants · CPC title