Soybean transgenic event MON87751 and methods for detection and use thereof

Patent metadata

Field	Value
Publication number	US-9719145-B2
Application number	US-201414303042-A
Country	US
Kind code	B2
Filing date	Jun 12, 2014
Priority date	Jun 14, 2013
Publication date	Aug 1, 2017
Grant date	Aug 1, 2017

Abstract

The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.

First claim

What is claimed is:

1. A recombinant DNA molecule detectable in a sample containing soybean DNA, wherein the nucleotide sequence of said molecule is:
a) selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10; or
b) a nucleotide sequence completely complementary to (a),
wherein the presence of such DNA molecule is diagnostic for soybean event MON87751 DNA in said sample.

2. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule is derived from soybean event MON87751, a representative sample of seed comprising soybean event MON87751 having been deposited under ATCC Accession No. PTA-120166.

3. A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with soybean event MON87751 DNA in a sample, wherein said DNA molecule comprises SEQ ID NO:1 or SEQ ID NO:2 and detecting hybridization of said DNA molecule under said hybridization conditions is diagnostic for soybean event MON87751 DNA in said sample.

4. The recombinant DNA molecule of claim 1 , wherein said sample comprises a soybean plant, soybean plant cell, soybean seed, soybean plant part, soybean progeny plant, soybean oil, soybean protein, or soybean commodity product.

5. A method of detecting the presence of a DNA segment diagnostic for soybean event MON87751 DNA in a sample, said method comprising:
a) contacting said sample with the DNA probe of claim 3 ;
b) subjecting said sample and said DNA probe to stringent hybridization conditions; and
c) detecting hybridization of said DNA probe to said sample,
wherein said detecting step is diagnostic for the presence of said soybean event MON87751 DNA in said sample.

6. A method of detecting the presence of a DNA segment diagnostic for soybean event MON87751 DNA in a sample, said method comprising:
a) contacting said sample with a pair of DNA molecules comprising a first DNA molecule and a second DNA molecule that is different from said first DNA molecule, wherein said first and second DNA molecules function as DNA primers when used together in an amplification reaction with a sample containing soybean event MON87751 DNA template DNA to produce a DNA amplicon that is diagnostic for said soybean event MON87751 DNA in said sample, and wherein said DNA amplicon comprises the nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2;
b) performing an amplification reaction sufficient to produce said DNA amplicon; and
c) detecting the presence of said DNA amplicon in said amplification reaction,
wherein said detecting the presence of said DNA amplicon is diagnostic for said soybean event MON87751 DNA in said sample.

7. A soybean plant, soybean plant part, or soybean cell thereof comprising the recombinant DNA molecule of claim 1 .

8. The soybean plant, soybean plant part, or soybean cell thereof of claim 7 , wherein said soybean plant, or soybean plant part or soybean cell is insecticidal when provided in the diet of a lepidopteran insect pest.

9. The soybean plant, soybean plant part, or soybean cell thereof of claim 8 , wherein said lepidopteran insect pest is selected from the group consisting of Chrysodeixis spp., Spodoptera spp., Helicoverpa spp., Crocidosema spp., Rachiplusia spp., Anticarsia spp., Elasmopalpus spp., and Plathypena spp.

10. The soybean plant, soybean plant part, or soybean cell thereof of claim 7 , wherein said soybean plant is further defined as a progeny of any generation of a soybean plant comprising said soybean event MON87751.

11. A method for protecting a soybean plant from insect infestation, wherein said method comprises providing in the diet of a lepidopteran pest of soybean an insecticidally effective amount of cells or tissue of a soybean plant comprising the recombinant DNA molecule of claim 1 .

12. The method of claim 11 , wherein said lepidopteran insect pest is selected from the group consisting of Chrysodeixis spp., Spodoptera spp., Helicoverpa spp., Crocidosema spp., Rachiplusia spp., Anticarsia spp., Elasmopalpus spp., and Plathypena spp.

13. A method of producing an insect resistant soybean plant, said method comprising:
a) sexually crossing two different soybean plants with at least one of the two different soybean plants comprising the recombinant DNA molecule of claim 1 ;
b) sampling seed or tissue from progeny of said crossing;
c) detecting the presence of a DNA segment diagnostic for soybean event MON87751 in the sample from step b) to identify progeny comprising soybean event MON87751 DNA; and
d) selecting said progeny comprising soybean event MON87751 DNA, wherein said progeny is an insect resistant soybean plant comprising transgenic soybean event MON87751 DNA.

14. A soybean seed comprising a detectable amount of the recombinant DNA molecule of claim 1 .

15. A nonliving soybean plant material comprising a detectable amount of the recombinant DNA molecule of claim 1 .

16. A microorganism comprising a detectable amount of the recombinant DNA molecule of claim 1 .

17. The microorganism of claim 16 , wherein said microorganism is a plant cell.

18. A soybean commodity product comprising a detectable amount of a DNA molecule unique for event MON87751, wherein said molecule comprises the recombinant DNA molecule of claim 1 .

19. The soybean commodity product of claim 18 , further defined as a commodity product selected from the group consisting of whole or processed soybean seeds, soybean oil, soybean protein, soybean meal, soybean flour, soybean flakes, soybean bran, soybean milk, soybean cheese, soybean wine, animal feed comprising soybean, paper comprising soybean, cream comprising soybean, soybean biomass, and fuel products produced using soybean plants and soybean plant parts.

20. A soybean plant, soybean plant part, or soybean seed thereof comprising DNA functional as a template when tested in a DNA amplification method producing an amplicon diagnostic for the presence of event MON87751 DNA, wherein said amplicon comprises the recombinant DNA molecule of claim 1 .

21. A method of determining the zygosity of a soybean plant or soybean seed comprising the recombinant DNA molecule of claim 1 , said method comprising:
a) contacting a sample comprising soybean DNA with a primer set capable of producing a first amplicon diagnostic for the presence of the recombinant DNA molecule of claim 1 and a second amplicon diagnostic for native soybean genomic DNA not comprising the recombinant DNA molecule of claim 1 ;
i) performing a nucleic acid amplification reaction with said sample and said primer set; and
ii) detecting in said nucleic acid amplification reaction said first amplicon diagnostic for the presence of the recombinant DNA molecule of claim 1 , or said second amplicon diagnostic for native soybean genomic DNA not comprising the recombinant DNA molecule of claim 1 ;
wherein the presence of only said first amplicon is diagnostic for homozygous alleles comprising the recombinant DNA molecule of claim 1 in said sample, and the presence of both said first amplicon and said second amplicon is diagnostic for heterozygous alleles in said sample, wherein one of said alleles comprises the recombinant DNA molecule of claim 1 ; or

b) contacting said sample comprising soybean DNA with a probe set that contains at least a first probe that spe

Assignees

• Monsanto Technology Llc
• Monsanto Technology Llc

Inventors

• Beazley Kim A
• Burns Wen C
• Cole Ii Robert H
• Macrae Ted C
• Miklos John A
• Ruschke Lisa G
• Tian Kairong
• Wei Liping
• Wu Kunsheng

Classifications

• C12Q2600/13

  Plant traits · CPC title

• A23K10/00

  Animal feeding-stuffs · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• A23J1/14

  from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds · CPC title

• C12Q2600/158

  Expression markers · CPC title