Maize event dp-004114-3 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-2019136331-A1
Application number	US-201916246608-A
Country	US
Kind code	A1
Filing date	Jan 14, 2019
Priority date	Dec 17, 2009
Publication date	May 9, 2019
Grant date	—

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

First claim

1 - 33 . (canceled)

34 . A method of detecting the presence of a nucleic acid molecule that is unique to event DP-004114-3 in a sample comprising corn nucleic acids, the method comprising:
(a) contacting the sample with a pair of primers that, when used in a nucleic-acid amplification reaction with genomic DNA from event DP-004114-3 produces an amplicon that is diagnostic for event DP-004114-3; (b) performing a nucleic acid amplification reaction, thereby producing the amplicon; and (c) detecting the amplicon.

35 . A pair of polynucleotide primers comprising a first polynucleotide primer and a second polynucleotide primer which function together in the presence of event DP-004114-3 DNA template in a sample to produce an amplicon diagnostic for event DP-004114-3.

36 . The pair of polynucleotide primers according to claim 35 , wherein the sequence of the first polynucleotide primer is or is complementary to a corn plant genome sequence flanking the point of insertion of a heterologous DNA sequence inserted into the corn plant genome of event DP-004114-3, and the sequence of the second polynucleotide primer is or is complementary to the heterologous DNA sequence inserted into the genome of event DP-004114-3.

37 . The pair of polynucleotide primers according to claim 36 , wherein
(a) the first polynucleotide primer comprises at least 10 contiguous nucleotides of a nucleotide sequence selected from the group consisting of nucleotides 1-2422 of SEQ ID NO: 6, nucleotides 14348-16752 of SEQ ID NO: 6, and the complements thereof; and (b) the second polynucleotide primer comprises at least 10 contiguous nucleotides from nucleotides 2423-14347 of SEQ ID NO: 6, or the complements thereof.

38 . The pair of polynucleotide primers according to claim 37 , wherein
(a) the first polynucleotide primer comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 20, SEQ ID NOs: 22-26 and the complements thereof; and (b) the second polynucleotide primer comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NOs: 14-19, SEQ ID NO: 21, and the complements thereof.

39 . The primer pair of claim 37 , wherein said first primer and said second primer are at least 18 nucleotides.

40 . The primer pair of claim 37 , wherein said first primer and said second primer are at least 24 nucleotides.

41 . A method of detecting the presence of DNA corresponding to the DP-004114-3 event in a sample, the method comprising:
(a) contacting the sample comprising maize DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from maize event DP-004114-3 and does not hybridize under said stringent hybridization conditions with a non-DP-004114-3 maize plant DNA; (b) subjecting the sample and probe to stringent hybridization conditions; and (c) detecting hybridization of the probe to the DNA;
wherein detection of hybridization indicates the presence of the DP-004114-3 event.

42 . A kit for detecting nucleic acids that are unique to event DP-004114-3 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event DP-004114-3 in the sample.

43 . The kit according to claim 42 , wherein the nucleic acid molecule comprises a nucleotide sequence from SEQ ID NO: 6.

44 . The kit according to claim 43 , wherein the nucleic acid molecule is a primer selected from the group consisting of SEQ ID NOs: 11-26, and the complements thereof.

Assignees

• Pioneer Hi Bred Int
• Du Pont

Inventors

• Diehn Scott
• Lu Albert L
• Nowatzki Timothy M
• Nubel Douglas S
• Pascual M Alejandra
• Register Iii James C
• Scelonge Christopher J
• Young Joshua K
• Zhong Cathy Xiaoyan
• Crowgey Erin L

Classifications

• C12Q2600/13

  Plant traits · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title