Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-9617553-B2
Application number	US-201414207302-A
Country	US
Kind code	B2
Filing date	Mar 12, 2014
Priority date	Mar 14, 2013
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

The invention provides recombinant DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising a recombinant DNA molecule comprising a DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of their use.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 13, wherein the sequence has gene-regulatory activity;
b) a DNA sequence comprising SEQ ID NO: 13; and
c) a fragment comprising at least 600 contiguous nucleotides of SEQ ID NO: 13, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

2. The recombinant DNA molecule of claim 1 , wherein said DNA sequence has the DNA sequence of SEQ ID NO: 13.

3. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.

4. The recombinant DNA molecule of claim 3 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

5. The recombinant DNA molecule of claim 3 , wherein the gene of agronomic interest confers pest resistance in plants.

6. A transgenic plant cell comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 13, wherein the sequence has gene-regulatory activity;
b) a DNA sequence comprising SEQ ID NO: 13; and
c) a fragment comprising at least 600 contiguous nucleotides of SEQ ID NO: 13, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

7. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a monocotyledonous plant cell.

8. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a dicotyledonous plant cell.

9. A transgenic plant, or part thereof, comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence with at least 99 percent sequence identity to the full length of SEQ ID NO: 13, wherein the sequence has gene-regulatory activity;
b) a DNA sequence comprising SEQ ID NO: 13; and
c) a fragment comprising at least 600 contiguous nucleotides of SEQ ID NO: 13, wherein the fragment has gene-regulatory activity;
wherein said sequence is operably linked to a heterologous transcribable polynucleotide molecule.

10. A progeny plant of the transgenic plant of claim 9 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

11. A transgenic seed of the transgenic plant of claim 9 , wherein the seed comprises the recombinant DNA molecule.

12. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 9 and producing the commodity product therefrom.

13. The method of claim 12 , wherein the commodity product is processed seeds, grains, plant parts, or meal.

14. A method of producing a transgenic plant comprising:
a) transforming a plant cell with the recombinant DNA molecule of claim 1 to produce a transformed plant cell; and
b) regenerating a transgenic plant from the transformed plant cell.

Assignees

• Monsanto Technology Llc

Inventors

• Flasinski Stanislaw
• Zhang Jun
• Zhao Suling

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title

• C12Y302/01056

  Glucuronosyl-disulfoglucosamine glucuronidase (3.2.1.56), i.e. glycuronidase · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title