Contact imaging of standards with illumination

Patent metadata

Field	Value
Publication number	US-12015751-B2
Application number	US-202218082330-A
Country	US
Kind code	B2
Filing date	Dec 15, 2022
Priority date	Dec 20, 2021
Publication date	Jun 18, 2024
Grant date	Jun 18, 2024

Abstract

Systems and methods for contact imaging of stands with illumination are disclosed herein. A contact imaging system can include a contact imager. The contact imager can include a housing having a base and a lid. The lid can have a closed position against the base and an open position. The contact imager can include a contact area image sensor. The lid can shield the contact area image sensor from ambient light when the lid is in the closed position. The contact imager can include an illuminator that can illuminate at least a portion of a blot on the contact area image sensor when the lid is in the closed position.

First claim

What is claimed is:

1. A contact imaging system, comprising:
a contact imager comprising:
a housing having a base and a lid, the lid having a closed position against the base and having an open position; and
a contact area image sensor located in the base, wherein the lid shields the contact area image sensor from ambient light when the lid is in the closed position; and

an illuminator configured to illuminate at least a portion of a blot on the contact area image sensor when the lid is in the closed position.

2. The contact imaging system of claim 1 , wherein the illuminator is a component of the contact imager.

3. The contact imaging system of claim 1 , wherein the illuminator is configured to illuminate at least the portion of the blot with visible light.

4. The contact imaging system of claim 3 , wherein the illuminator comprises a 2-D LED array.

5. The contact imaging system of claim 4 , wherein the 2-D LED array is located in the lid of the contact imager.

6. The contact imaging system of claim 4 , wherein the 2-D LED array is configured to generate illumination at at least one desired frequency.

7. The contact imaging system of claim 3 , wherein the illuminator comprises a side-lit assembly located in the contact imager.

8. The contact imaging system of claim 7 , wherein the side-lit assembly is located in the lid of the contact imager.

9. The contact imaging system of claim 8 , wherein the side-lit assembly comprises:
at least one Light emitting diode (LED) strip comprising a plurality of LEDs; and
a total internal reflection (TIR) layer configured to cover the contact area image sensor when the lid is in the closed position, wherein the TIR layer is configured to receive light from the LED strip and distribute this light across the contact area image sensor.

10. The contact imaging system of claim 9 , wherein the side-lit assembly further comprises:
a reflecting layer coupled to a top of the TIR layer; and
at least one distribution layer coupled to a bottom of the TIR layer such that the at least one distribution layer is between the TIR layer and the contact area image sensor when the lid is in the closed position.

11. The contact imaging system of claim 10 , wherein the at least one distribution layer comprises a diffusion layer.

12. The contact imaging system of claim 11 , wherein the at least one distribution layer further comprises a prism layer having a first orientation, and a second prism layer having a second orientation.

13. The contact imaging system of claim 12 , wherein the first orientation is orthogonal to the second orientation.

14. The contact imaging system of claim 3 , further comprising at least one processor configured to:
generate a first image of first sample features with the contact area image sensor, wherein the first image is of a resolved sample and is generated with light emitted by chemiluminescence of the sample;
generate a second image of second sample features with the contact area image sensor; the second sample features comprising standards.

15. The contact imaging system of claim 14 , wherein the first image and the second image are the same image.

16. The contact imaging system of claim 14 , wherein the first image and the second image are separate images captured at different times.

17. The contact imaging system of claim 16 , the at least one processor can be further configured to combine the first image and the second image.

18. The contact imaging system of claim 14 , wherein the second image is generated with light from the illuminator transilluminating the sample.

19. The contact imaging system of claim 18 , wherein the at least one processor is further configured to control the illuminator to transilluminate the sample before generating the second image.

20. A method of imaging a sample, the method comprising:
placing a resolved sample on a contact area image sensor, the contact area image sensor located in a base of a contact imager;
moving a lid of the contact area image sensor to a closed position, wherein in the closed position, the lid prevents ambient light from reaching the contact area image sensor;
generating, with the contact area image sensor, a first image of first sample features, wherein the first image is of the resolved sample and is generated with light emitted by the sample;
illuminating the resolved sample with an illuminator of the contact imager, the illuminator configured to illuminate at least a portion of the contact area image sensor when the lid is in the closed position; and
generating, with the contact area image sensor, a second image of second sample features while the lid is in the closed position, the second sample features comprising standards, wherein the second image of the second sample features is generated while the resolved sample is illuminated.

21. The method of claim 20 , wherein the sample is electrophoretically resolved, and wherein the light emitted by the sample is generated via chemiluminescence.

22. The method of claim 21 , wherein the illuminator transilluminates the sample.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Swihart Stephen
• Thrush Evan
• Marlowe Carl
• Oh Kenneth
• Griffin Michael

Classifications

• G01N27/44721

  by optical means · CPC title

• G01N21/76

  Chemiluminescence; Bioluminescence · CPC title

• G06T5/50

  using two or more images, e.g. averaging or subtraction · CPC title

• G06V10/141

  Control of illumination · CPC title

• G06T2207/20221

  Image fusion; Image merging · CPC title