Contact imaging devices for fluorescence applications

Patent metadata

Field	Value
Publication number	US-9933565-B2
Application number	US-201615211728-A
Country	US
Kind code	B2
Filing date	Jul 15, 2016
Priority date	Jul 17, 2015
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

Devices, systems, methods, and kits for contact imaging are provided. A contact imaging device includes an imaging sensor, a fixed fiber faceplate mechanically coupled to the imaging sensor, and an optical filtering layer mechanically coupled to the fixed fiber faceplate. The optical filtering layer can include an interference filter, an absorptive filter, and/or a removable fiber faceplate. The contact imaging device can be used to image fluorescent samples by filtering out excitation light on the basis of wavelength and/or angle of incidence.

First claim

What is claimed is:

1. A contact imaging device comprising:
an imaging sensor;
a fixed fiber faceplate directly mechanically coupled to the imaging sensor, wherein the fixed fiber faceplate comprises a plurality of optical fibers and an absorptive material, wherein the absorptive material of the fixed fiber faceplate comprises a plurality of black fibers interspersed among the plurality of optical fibers, disposed in interstitial spaces between the optical fibers; and
an optical filtering layer mechanically coupled to the fixed fiber faceplate, wherein the optical filtering layer further comprises a removable fiber faceplate and a transmission filter, wherein the transmission filter is an interference filter or an absorptive filter, wherein the removable fiber faceplate is bonded on one side with the transmission filter, and wherein the optical filtering layer is configured to be removed from the fixed fiber faceplate.

2. The contact imaging device of claim 1 , wherein the optical filtering layer comprises an interference filter, wherein the interference filter is configured to be a long-pass filter, to block ultraviolet light, or to pass visible light, or a combination thereof.

3. The contact imaging device of claim 2 , wherein the optical filtering layer comprises an absorptive filter, and wherein the absorptive filter is disposed between the interference filter and the fixed fiber faceplate.

4. The contact imaging device of claim 1 , wherein the absorptive material further comprises the core or the cladding of at least one of the optical fibers.

5. The contact imaging device of claim 1 , wherein the numerical aperture of the removable fiber faceplate is less than that of the fixed fiber faceplate.

6. The contact imaging device of claim 1 , wherein the removable fiber faceplate is disposed between the fixed fiber faceplate and the transmission filter, or wherein the transmission filter is disposed between the fixed fiber faceplate and the removable fiber faceplate.

7. The contact imaging device of claim 1 , wherein the fixed fiber faceplate is a fiber optic taper.

8. The contact imaging device of claim 1 , wherein the angle of acceptance of optical fibers of the fixed fiber faceplate is at most about 30 degrees.

9. The contact imaging device of claim 1 , wherein the black fibers have circular or hexagonal cross-sections.

10. The contact imaging device of claim 1 , wherein the plurality of black fibers are at a ratio of the number of black fibers to the number of optical fibers from 1:61 to 60:61.

11. The contact imaging device of claim 1 , wherein the plurality of black fibers occupy about 10 percent of the cross-sectional area of the fixed fiber faceplate.

12. A system for imaging a fluorescent, phosphorescent, or chemiluminescent sample, the system comprising:
an excitation light source; and
a contact imaging device, the contact imaging device comprising:
an imaging sensor;
a fixed fiber faceplate mechanically coupled to the imaging sensor, wherein the fixed fiber faceplate comprises a plurality of optical fibers and a plurality of absorptive material fibers disposed in interstitial spaces between the plurality of optical fibers;
an optical filtering layer mechanically coupled to the fixed fiber faceplate; and
an excitation fiber faceplate, wherein the excitation fiber faceplate is disposed between the excitation light source and the sample.

13. The system of claim 12 , further comprising a cassette, wherein the cassette comprises a base plate and a light-tight lid, and the contact imaging device is mechanically coupled to the base plate, wherein the base plate comprises a thermally conductive metal slab, and the imaging sensor is coupled to the metal slab.

14. The system of claim 12 , wherein light emitted by the excitation light source and transmitted by the excitation fiber faceplate is collimated.

15. The system of claim 12 , wherein the excitation light source is positioned relative to the contact imaging device such that light emitted by the excitation light source is not incident on the optical filtering layer.

16. The system of claim 12 , wherein the excitation light source is positioned relative to the contact imaging device such that light emitted by the excitation light source is incident on the optical filtering layer at an angle of at least about 30 degrees from the normal.

17. The system of claim 12 , wherein the excitation light source is positioned relative to the contact imaging device such that the excitation light source emits light in a direction parallel to the surface of the optical filtering layer.

18. The system of claim 12 , wherein the system is adapted to run one any one or a combination of a Western blot, a dot blot, a Northern blot, or a Southern blot.

19. The system of claim 12 , wherein the surface of the excitation fiber faceplate arranged to face the excitation light source has a numerical aperture of at most 1.0.

20. The system of claim 12 , wherein the absorptive material fibers have circular or hexagonal cross-sections.

21. The system of claim 12 , wherein the plurality of absorptive material fibers are present at a ratio of the number of absorptive material fibers to the number of optical fibers from 1:61 to 60:61.

22. The system of claim 12 , wherein the plurality of absorptive material fibers occupy about 10 percent of the cross-sectional area of the fixed fiber faceplate.

Assignees

• Bio Rad Laboratories

Inventors

• Swihart Steve
• Thrush Evan
• Uri Yochanan
• Ran Boaz
• Barak Itay
• Mcdonald Kevin

Classifications

• G01N27/44721

  by optical means · CPC title

• G01N2021/6484

  Optical fibres · CPC title

• G02B5/28

  Interference filters · CPC title

• G01N21/6454 Primary

  using an integrated detector array · CPC title

• G02B6/08 Primary

  with fibre bundle in form of plate · CPC title