Method of reading the result of an electrophoretic assay comprising a digital image indicating the intensity of light emitted by chemiluminescence from the output medium of the electrophoretic assay

Patent metadata

Field	Value
Publication number	US-11035722-B2
Application number	US-201816219715-A
Country	US
Kind code	B2
Filing date	Dec 13, 2018
Priority date	Dec 15, 2017
Publication date	Jun 15, 2021
Grant date	Jun 15, 2021

Abstract

A combination imaging system includes a housing having a base and a lid, the lid having a closed position against the base and having an open position. The imaging device further includes a contact area image sensor. The lid shields the contact area image sensor from ambient light when the lid is in the closed position. The imaging device also includes a camera. The camera includes a lens, and the field of view of the camera encompasses at least a portion of an imaging area of the contact area image sensor when the lid is in the open position. The device may be especially useful for capturing a chemiluminescent image of an electrophoretic assay result, and capturing a colorimetric image of the same result, so that non-chemiluminescent protein standards may be located with respect to chemiluminescent analytes of interest.

First claim

What is claimed is:

1. A method of reading the result of an electrophoretic assay, the method comprising:
placing an output medium of the electrophoretic assay on a contact area image sensor of a combination imaging device;
closing a lid of the combination imaging device, blocking ambient light from reaching the contact area image sensor;
capturing a first digital image of the output medium using the contact area image sensor, the first digital image indicating the intensity of light emitted by chemiluminescence from the output medium;
opening the lid;
capturing a second digital image of the output medium using a camera mounted in the lid, the field of view of the camera encompassing at least a portion of the output medium when the lid is in an open position, wherein the second digital image indicates the intensity of light reflecting from the output medium; and
combining the first and second digital images into a composite digital image.

2. The method of claim 1 , wherein:
the output medium is a blot, the blot carrying proteins tagged to emit light by chemiluminescence and the blot carrying protein standards that are not chemiluminescent but are visible the second digital image; and
combining the first and second digital images into a composite digital image comprises combining the first and second digital images into a digital image in which both the chemiluminescence from the tagged proteins and the non-chemiluminescent protein standards are shown.

3. The method of claim 1 , wherein:
the first digital image is captured before the second digital image is captured; or
the second digital image is captured before the first digital image is captured.

4. The method of claim 1 , wherein the output medium is a membrane.

5. The method of claim 1 , wherein the combination imaging device does not include a light source for providing illumination for the second digital image, and wherein capturing the second digital image comprises capturing the second digital image using ambient light.

6. The method of claim 1 , further comprising providing supplemental light to the output medium using a light source in the combination imaging device, wherein capturing the second digital image comprises capturing the second digital image using the supplemental light.

7. The method of claim 1 , further comprising digitally adjusting the size of at least one of the first and second digital images so that the first and second digital images are of the same size.

8. The method of claim 1 , further comprising digitally removing distortion from at least one of the first and second digital images.

9. The method of claim 1 , further comprising:
sensing whether the lid is in the closed position or an open position; and
instructing a user of the imaging device to open or close the lid.

10. The method of claim 1 , further comprising:
placing a gel on the contact area image sensor; and
capturing one or more images of the gel using the contact area image sensor, the camera, or both.

11. A method of reading the result of an electrophoretic assay, the method comprising:
placing an output medium of the electrophoretic assay on a contact area image sensor of a combination imaging device;
covering a phosphorescent area in the lid of the combination image device;
capturing a first digital image of the output medium using the contact area image sensor, the first digital image indicating the intensity of light emitted by chemiluminescence from the output medium, wherein the first digital image is captured with the lid of the combination imaging device closed, blocking ambient light from reaching the contact area image sensor;
uncovering the phosphorescent area in the lid of the combination imaging device;
capturing a second digital image of the output medium using the contact area image sensor, wherein the second digital image indicates the intensity of light passing through the output medium from the phosphorescent area, wherein the second digital image is captured with the lid of the combination imaging device closed, blocking ambient light from reaching the contact area image sensor; and
combining the first and second digital images into a composite digital image.

Assignees

• Bio Rad Laboratories

Inventors

• Barak Itay

Classifications

• G01N27/44726

  using specific dyes, markers or binding molecules · CPC title

• G01N21/76 Primary

  Chemiluminescence; Bioluminescence · CPC title

• G01N21/6456 Primary

  Spatial resolved fluorescence measurements; Imaging · CPC title

• A61B5/0071

  by measuring fluorescence emission · CPC title

• G01J1/0403 Primary

  Mechanical elements; Supports for optical elements; Scanning arrangements · CPC title