Sample dispenser including an internal standard and methods of use thereof

Patent metadata

Field	Value
Publication number	US-11867684-B2
Application number	US-202217584100-A
Country	US
Kind code	B2
Filing date	Jan 25, 2022
Priority date	Apr 30, 2009
Publication date	Jan 9, 2024
Grant date	Jan 9, 2024

Abstract

The invention generally relates to a sample dispenser including an internal standard and methods of use thereof.

First claim

What is claimed is:

1. A method for analyzing a sample comprising:
preparing a mixture of a sample with a controlled amount of an internal standard;
contacting the mixture to a porous substrate that tapers to at least one tip, wherein the porous material is kept separate from a flow of solvent and the probe operates without pneumatic assistance;
applying a high voltage to the porous material such that an electric field is generated at a tip of the material so as to generate ions of an analyte and ions of the internal standard that are expelled from the porous material; and
analyzing the expelled ions.

2. The method according to claim 1 , further comprising applying a solvent to the porous substrate.

3. The method according to claim 1 , wherein the solvent assists in transport of the sample through the porous substrate.

4. The method according to claim 2 , wherein the solvent minimizes salt and matrix effects.

5. The method according to claim 1 , wherein analyzing comprises providing a mass analyzer to generate a mass spectrum of analytes in the sample.

6. The method according to claim 1 , wherein the sample is a biological sample.

7. The method according to claim 6 , wherein the biological sample is a blood sample or a urine sample.

8. The method according to claim 1 , further comprising quantifying a target analyte in the sample.

9. The method according to claim 1 , wherein the preparing step comprises introducing the sample to a capillary on which walls of the capillary comprise the internal standard.

10. The method according to claim 9 , wherein a volume of the capillary regulates a volume of the sample and a volume of the internal standard that comprise the mixture.

11. The method according to claim 9 , wherein the sample comprises a volume of about 1 μL or less.

Assignees

• Purdue Research Foundation

Inventors

• Ouyang Zheng
• Wang He
• Manicke Nicholas E
• Cooks Robert Graham
• Yang Qian
• Liu Jiangjiang

Classifications

• G01N33/492 Primary

  Determining multiple analytes · CPC title

• G01N1/28 Primary

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• G01N1/286

  involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F) · CPC title

• G01N33/48714

  for determining substances foreign to the organism, e.g. drugs or heavy metals (drugs by chemical analysis G01N33/94) · CPC title

• G01N33/48785

  Electrical and electronic details of measuring devices for physical analysis of liquid biological material not specific to a particular test method, e.g. user interface or power supply · CPC title