Mass spectrometry analysis of microorganisms in samples

Patent metadata

Field	Value
Publication number	US-9035239-B1
Application number	US-201514594233-A
Country	US
Kind code	B1
Filing date	Jan 12, 2015
Priority date	Apr 30, 2009
Publication date	May 19, 2015
Grant date	May 19, 2015

Abstract

The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

First claim

What is claimed is:

1. A method for analyzing a mammalian sample for presence of a microorganism, the method comprising:
obtaining a mammalian sample comprising a microorganism;
contacting the sample to a porous material;
generating ions of the microorganism from the porous material; and
analyzing the ions, thereby analyzing the sample for the presence of the microorganism.

2. The method according to claim 1 , wherein the porous material is kept separate from a flow of solvent.

3. The method according to claim 1 , wherein generating comprises applying voltage to the porous material.

4. The method according to claim 1 , further comprising applying a solvent to the porous material.

5. The method according to claim 1 , wherein the mammalian sample is a human sample.

6. The method according to claim 1 , wherein the human sample is a human tissue or human body fluid.

7. The method according to claim 1 , wherein the porous material is paper or PVDF membrane.

8. The method according to claim 1 , wherein the microorganism is selected from the group consisting of a bacterium, a virus, a protozoan, and a fungus.

9. The method according to claim 1 , wherein analyzing uses a mass spectrometer.

10. The method according to claim 9 , wherein the mass spectrometer is a miniature mass spectrometer.

11. A method for analyzing a mammalian sample for presence of a microorganism, the method comprising:
contacting a mammalian sample comprising a microorganism to a porous material;
generating one or more mass spectra using a mass spectrometer;
comparing the one or more mass spectra to a database that comprises mass spectra of known organisms, wherein a match between a mass spectra generated from the sample with a mass spectra in the database identifies the presence of a microorganism in the sample.

12. The method according to claim 11 , wherein the porous material is kept separate from a flow of solvent.

13. The method according to claim 11 , wherein generating comprises applying solvent and a high voltage to the probe.

14. The method according to claim 11 , wherein the porous material is paper or PVDF membrane.

15. The method according to claim 11 , wherein the mammalian sample is a human sample.

16. The method according to claim 15 , wherein the human sample is a human tissue or human body fluid.

17. The method according to claim 11 , wherein the mass spectrometer is a miniature mass spectrometer.

18. The method according to claim 11 , wherein the mass spectrometer is selected from the group consisting of: a quadrupole ion trap, a rectalinear ion trap, a cylindrical ion trap, a ion cyclotron resonance trap, and an orbitrap.

19. The method according to claim 11 , wherein the microorganism is selected from the group consisting of a bacterium, a virus, a protozoan, and a fungus.

20. The method of claim 11 , wherein the database comprises a mass spectrum from a genus, a species, a strain, a sub-strain, or an isolate of organisms.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Hamid Ahmed Mohamed
• Jarmusch Alan Keith
• Ouyang Zheng

Classifications

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• H01J49/0409

  Sample holders or containers (containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543) · CPC title

• H01J49/0422

  for gaseous samples (interfaces to gas chromatographs G01N30/7206) · CPC title

• H01J49/10

  Ion sources; Ion guns · CPC title