Ion generation using modified wetted porous materials

Patent metadata

Field	Value
Publication number	US-9500630-B2
Application number	US-201614987154-A
Country	US
Kind code	B2
Filing date	Jan 4, 2016
Priority date	Jun 3, 2011
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

The invention generally relates to ion generation using modified wetted porous materials. In certain aspects, the invention generally relates to systems and methods for ion generation using a wetted porous substrate that substantially prevents diffusion of sample into the substrate. In other aspects, the invention generally relate to ion generation using a wetted porous material and a drying agent. In other aspects, the invention generally relates to ion generation using a modified wetted porous substrate in which at least a portion of the porous substrate includes a material that modifies an interaction between a sample and the substrate.

First claim

What is claimed is:

1. A system for analyzing a sample material comprising:
at least one porous substrate connected to a voltage source, wherein the porous substrate is coated with a material that substantially prevents diffusion of a sample into the substrate in a manner that the sample remains substantially on top of the substrate until a solvent is applied to the substrate; and
a mass analyzer.

2. The system according to claim 1 , wherein the porous substrate is discrete from a flow of solvent.

3. The system according to claim 1 , wherein the porous substrate is silanized paper.

4. The system according to claim 1 , further comprising a solvent applied to the substrate, wherein the solvent is capable of diffusing into the substrate.

5. The system according to claim 4 , wherein the solvent assists transport of the sample through the substrate.

6. The system according to claim 5 , wherein the solvent contains an internal standard.

7. The system according to claim 1 , wherein the mass analyzer is for a mass spectrometer or a handheld mass spectrometer.

8. A method for analyzing a sample comprising:
contacting a sample to a porous substrate, wherein the porous substrate is coated with a material that substantially prevents diffusion of a sample into the substrate in a manner that the sample remains substantially on top of the substrate until a solvent is applied to the substrate;
applying a solvent that is capable of diffusing into the substrate to the sample, resulting in diffusion of components of the sample into the substrate;
applying a voltage to the substrate to generate ions of the components that are expelled from the porous substrate; and
analyzing the expelled ions.

9. The method according to claim 8 , wherein the porous substrate is kept separate from a flow of solvent.

10. The method according to claim 8 , wherein the sample is hydrophilic and the substrate is less hydrophilic than the sample.

11. The method according to claim 8 , wherein the sample is hydrophobic and the substrate is less hydrophobic than the sample.

12. The method according to claim 8 , wherein the sample is a biological fluid.

13. The method according to claim 12 , wherein the biological fluid is blood.

14. The method according to claim 8 , wherein the solvent assists in transport of the sample through the porous substrate.

15. The method according to claim 8 , wherein the solvent comprises an internal standard.

16. The method according to claim 8 , wherein the solvent is capable of mixing with the sample.

17. The method according to claim 8 , wherein the solvent is not capable of mixing with the sample but is capable of extracting the components from the sample.

18. The method according to claim 8 , wherein prior to applying the voltage, the method further comprises drying the substrate.

19. The method according to claim 8 , wherein analyzing comprises providing a mass analyzer to generate a mass spectrum of analytes in the sample.

20. A method for analyzing blood, the method comprising:
contacting a blood sample to a porous substrate, wherein the porous substrate is coated with a material that substantially prevents diffusion of a sample into the substrate in a manner that the sample remains substantially on top of the substrate until a solvent is applied to the substrate;
applying to the blood sample a solvent that is capable of diffusing into the substrate, resulting in diffusion of components of the blood sample into the substrate;
applying a voltage to the substrate to generate ions of the components that are expelled from the porous substrate; and
analyzing the expelled ions.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Ouyang Zheng
• Wang He

Classifications

• H01J49/168 Primary

  field ionisation, e.g. corona discharge (atmospheric pressure corona discharge per se H01T19/00) · CPC title

• G01N30/91

  Application of the sample · CPC title

• H01J49/0431

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title

• G01N30/95 Primary

  Detectors specially adapted therefor; Signal analysis · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title