Method for producing cell extract having agricultural pest insecticidal capacity and method for insecticide of agricultural pest
US-2016345587-A1 · Dec 1, 2016 · US
US11859226B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11859226-B2 |
| Application number | US-201716603213-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 26, 2017 |
| Priority date | Jun 2, 2017 |
| Publication date | Jan 2, 2024 |
| Grant date | Jan 2, 2024 |
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The present invention relates to a newly isolated bacterium belonging to the genus Microbacterium , a composition for producing psicose comprising the strain, and a method for producing psicose using the same.
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The invention claimed is: 1. A method for producing a psicose from a fructose-containing substrate using a bacterium belonging to the genus Microbacterium having a psicose conversion activity, comprising reacting the fructose-containing substrate with at least one selected from the group consisting of a microbial cell of the bacterium, a culture of the bacterium, and a lysate of the bacterium in the presence of at least one metal ion selected from the group consisting of manganese ion and cobalt ion, wherein the bacterium belonging to the genus Microbacterium is Microbacterium phyllosphaerae, wherein the Microbacterium phyllosphaerae is a strain deposited as an accession number KCCM12034P. 2. The method for producing a psicose of claim 1 , wherein the step of reacting is performed by contacting the fructose-containing substrate with a support immobilized with at least one selected from the group consisting of a microbial cell of the bacterium, a culture of the bacterium, and a lysate of the bacterium. 3. The method for producing a psicose of claim 1 , wherein the fructose-containing substrate contains fructose at a concentration of 40 to 75% (w/w). 4. The method for producing a psicose of claim 1 , wherein the method is characterized by not using a buffer solution. 5. The method for producing a psicose of claim 1 , wherein the method is performed under the temperature condition of 40 to 80° C. 6. The method for producing a psicose of claim 1 , wherein the method is performed for 1 to 48 hours.
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