Production of proteins in filamentous fungi

Patent metadata

Field	Value
Publication number	US-9499826-B2
Application number	US-201113701933-A
Country	US
Kind code	B2
Filing date	May 30, 2011
Priority date	Jun 4, 2010
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

First claim

The invention claimed is:

1. A method to genetically modify a Trichoderma reesei host for modified protein production, said method comprising:
genetically modifying the host to be deficient of, with reduced or lacking amount or activity, or to overexpress, with increased amount or activity of gene tre108381 (SEQ ID NO:1) or coding region of SEQ ID NO:1, said host being capable of increased or decreased production of cellulase, hemicellulase or a protein that is expressed under a cellulase or a hemicellulase promoter.

2. The method according to claim 1 , wherein the host is genetically modified to be deficient of gene tre108381 (SEQ ID NO:1) or coding region of SEQ ID NO:1, said host being capable of increased production of cellulase, hemicellulase, or a protein expressed under a cellulase or a hemicellulase promoter.

3. The method according to claim 1 , wherein the host is genetically modified to overexpress gene tre108381 (SEQ ID NO:1) or coding region of SEQ ID NO:1, said host being capable of decreased production of cellulase, hemicellulase, or a protein expressed under a cellulase or a hemicellulase promoter.

4. A Trichoderma reesei host genetically modified to be deficient of or to overexpress gene [t]tre108381 (SEQ ID NO:1) or coding region of SEQ ID NO: 1, said host being capable of modified production of cellulases, hemicellulases, or a protein expressed under a cellulase or a hemicellulase promoter.

5. The host according to claim 4 , wherein the host is genetically modified to be deficient of gene tre108381 (SEQ ID NO:1) or coding region of SEQ ID NO:1, said host being capable of increased production of cellulase, hemicellulase, or a protein expressed under a cellulase or a hemicellulase promoter.

6. The host according to claim 4 , wherein the host is genetically modified to overexpress gene tre108381 (SEQ ID NO:1) or coding sequence of SEQ ID NO:1, said host being capable of decreased production of cellulase, hemicellulase, or a protein expressed under a cellulase or a hemicellulase promoter.

7. A method for improved production or for producing a modified composition of proteins in a Trichoderma reesei host, said method comprising a step of cultivating the modified filamentous fungus host of claim 4 under suitable culture conditions for protein production.

8. The method according to claim 7 , wherein the protein is selected from the group consisting of cellulases, hemicellulases, and a protein expressed under a cellulase or a hemicellulase promoter.

9. The method according to claim 7 , wherein the protein expressed under a cellulase or a hemicellulase promoter is a heterologous or recombinant protein.

Assignees

• Pakula Tiina
• Saloheimo Markku
• Hakkinen Mari
• Westerholm-Parvinen Ann
• Penttila Merja
• Vitikainen Marika
• Teknologian Tutkimuskeskus Vtt Oy

Inventors

• Pakula Tiina
• Saloheimo Markku
• Hakkinen Mari
• Westerholm-Parvinen Ann
• Penttila Merja
• Vitikainen Marika

Classifications

• C12N9/16

  acting on ester bonds (3.1) · CPC title

• C12N15/80 Primary

  for fungi · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• C07K14/37

  from fungi · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title