Encapsulation system for protection of probiotics during processing
US-9788563-B2 · Oct 17, 2017 · US
US11845927B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11845927-B2 |
| Application number | US-201916689978-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 20, 2019 |
| Priority date | Nov 21, 2018 |
| Publication date | Dec 19, 2023 |
| Grant date | Dec 19, 2023 |
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Certain disclosed embodiments concern a bioremediation composition comprising microbial cells, at least one co-metabolism substrate to induce selected enzyme production by the microbial cells, and a bead or gel encapsulating the microbial cells, such as bacterial or fungi cells, and the at least one co-metabolism substrate. For certain embodiments, the substrate is a slow release compound, such as an orthosilicate that hydrolyzes to produce an alcohol growth substrate. Embodiments of a method for using the composition to transform contaminants of concern also are disclosed.
Opening claim text (preview).
We claim: 1. A composition for remediating 1,4-dioxane, 1,1,1-trichloroethane (1,1,1-TCA) and/or dichloroethene (DCE), comprising: microbial cells obtained from Rhodococcus rhodochrous ATCC 21198; at least one orthosilicate co-metabolism substrate selected from tetrabutyl orthosilicate (TBOS), tetra-sec-butyl orthosilicate (T2BOS), and combinations thereof, to induce selected enzyme production by the microbial cells; and a bead having at least one dimension of at least 1 millimeter or greater encapsulating the microbial cells and the at least one co-metabolism substrate. 2. A method for remediating 1,4-dioxane, 1,1,1-TCA and/or DCE, comprising: providing a composition according to claim 1 ; and contacting 1,4-dioxane, 1,1,1-TCA and/or DCE with the composition.
the carrier being a carbohydrate · CPC title
without C-silicon linkages · CPC title
Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title
Bacteria; Culture media therefor · CPC title
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
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