Method and apparatus for culture of anaerobic ammonium oxidizer

Patent metadata

Field	Value
Publication number	US-2016176743-A1
Application number	US-201514840664-A
Country	US
Kind code	A1
Filing date	Aug 31, 2015
Priority date	Dec 22, 2014
Publication date	Jun 23, 2016
Grant date	—

Abstract

The present disclosure provides an apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria, including: a reactor containing a culture medium containing ammonium and nitrite; and activated sludge beads in which the anaerobic ammonium-oxidizing bacteria in the culture medium are supported, wherein the amount of the activated sludge beads is 25-35% based on the volume of the culture medium. Because loss of the anaerobic ammonium-oxidizing bacteria is minimized and the beads have good mechanical strength, the apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria can be operated for a long time.

First claim

What is claimed is:

1 . An apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria, comprising:
a reactor comprising a culture medium comprising ammonium and nitrite; and activated sludge beads in which the anaerobic ammonium-oxidizing bacteria in the culture medium are supported, wherein the amount of the activated sludge beads is 25-35% based on the volume of the culture medium.

2 . The apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria according to claim 1 , wherein the reactor is operated as a completely stirred tank reactor (CSTR) and the total nitrogen inflow rate in the reactor is controlled to be 0.8-1.5 kg N/m 3 ·day.

3 . The apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria according to claim 2 , wherein the reactor is operated as a sequencing batch reactor (SBR) and the total nitrogen concentration in the culture medium in the reactor is maintained at 120-180 mg N/L.

4 . The apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria according to claim 3 , wherein the pH of the culture medium is 6.5-8.

5 . The apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria according to claim 1 , wherein the activated sludge beads are formed by mixing and hardening the PVA (poly(vinyl alcohol)), the alginate and the activated sludge.

6 . The apparatus for enrichment culturing of anaerobic ammonium-oxidizing bacteria according to claim 1 , wherein a filtering sieve preventing outflow of the activated sludge beads is provided on top of the reactor.

Assignees

• Korea Inst Sci & Tech

Inventors

• Bae Hyokwan
• Lee Seockheon
• Chung Yun Chul
• Jung Jin-Young
• Yang Hee Jeong

Classifications

• C02F11/04 Primary

  Anaerobic treatment; Production of methane by such processes · CPC title

• C12N11/084

  Polymers containing vinyl alcohol units · CPC title

• C12M1/12

  with sterilisation, filtration or dialysis means · CPC title

• C12N1/00 Primary

  Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor · CPC title

• C12N11/10

  the carrier being a carbohydrate · CPC title