Co-encapsulation of live cells with oxygen-generating particles

Patent metadata

Field	Value
Publication number	US-9393272-B2
Application number	US-201214237214-A
Country	US
Kind code	B2
Filing date	Aug 9, 2012
Priority date	Aug 9, 2011
Publication date	Jul 19, 2016
Grant date	Jul 19, 2016

Abstract

Microcapsules are described that comprise (a) a liquid aqueous or hydrogel core; (b) a semipermeable membrane surrounding said core; (c) live animal cells (e.g., pancreatic cells) in the core; and (d) oxygen-generating particles in said core, said oxygen-generating particles included in said microcapsules in an amount sufficient to lengthen the duration of viability of said animal cells in said microcapsules. Compositions comprising such microcapsules and uses thereof, such as in treating diabetes, are also described.

First claim

That which is claimed is:

1. A microcapsule comprising:
(a) a liquid aqueous or hydrogel core;
(b) a semipermeable membrane layer surrounding said core;
(c) live mammalian cells in said core, said cells are human pancreatic islet cells;
(d) oxygen-generating particles in said core, said oxygen-generating particles included in said microcapsules in an amount sufficient to lengthen the duration of viability of said animal cells in said microcapsules; wherein said oxygen-generating particles comprise (i) magnesium peroxide, optionally in combination with a biodegradable polymer comprising alginate, and (ii) catalase;
(e) an antioxidant, which is trolox; and
(f) an auxiliary layer surrounding said semipermeable membrane layer, wherein said auxiliary layer comprises a biodegradable polymer comprised of sodium alginate, said biodegradable polymer containing an angiogenic agent in an amount effective to enhance the growth of blood vessels adjacent said microcapsule.

2. The microcapsule of claim 1 , wherein
said angiogenic agent is vascular endothelial growth factor.

Assignees

• Opara Emmanuel C
• Harrison Benjamin S
• Univ Wake Forest Health Sciences

Inventors

• Opara Emmanuel C
• Harrison Benjamin S

Classifications

• A61K31/555

  containing heavy metals, e.g. hemin, hematin, melarsoprol · CPC title

• A61K9/5036

  Polysaccharides, e.g. gums, alginate; Cyclodextrin · CPC title

• C12N11/10

  the carrier being a carbohydrate · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K35/39 Primary

  Pancreas; Islets of Langerhans (Langerhans cells of epidermis A61K35/36) · CPC title