Mass spectrometry analysis of microorganisms in samples

Patent metadata

Field	Value
Publication number	US-11830716-B2
Application number	US-202217841141-A
Country	US
Kind code	B2
Filing date	Jun 15, 2022
Priority date	Jun 25, 2013
Publication date	Nov 28, 2023
Grant date	Nov 28, 2023

Abstract

The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

First claim

What is claimed is:

1. A method for capturing an unknown microorganism from a sample, the method comprising:
providing a product comprising a capture module configured to capture an intact unknown microorganism from a sample, wherein the capture module comprises a porous substrate that is held between a top and bottom portion of a cartridge, the top and bottom portion sandwiching the porous substrate and each having an opening therein such that a portion of the porous substrate is exposed when the porous substrate in held in the cartridge; and
moving air or liquid through the exposed portion of the porous substrate such that any unknown microorganism in the air or liquid is retained and captured by the porous substrate.

2. The method according to claim 1 , wherein the air or liquid comprises a plurality of unknown microorganisms.

3. The method according to claim 1 , wherein the porous substrate is discrete from a flow of solvent.

4. The method according to claim 1 , wherein the porous substrate tapers to at least one distal tip.

5. The method according to claim 1 , further comprising a solvent applied to the porous substrate, wherein optionally, the solvent comprises an internal standard.

6. The method according to claim 1 , wherein the microorganism is selected from the group consisting of a bacterium, a virus, a protozoan, and a fungus.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Hamid Ahmed Mohamed
• Jarmusch Alan Keith
• Ouyang Zheng

Classifications

• H01J49/0445 Primary

  with means for introducing as a spray, a jet or an aerosol (electrospray ion sources H01J49/165) · CPC title

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• H01J49/16

  using surface ionisation, e.g. field-, thermionic- or photo-emission · CPC title