Protein quantitation device

Patent metadata

Field	Value
Publication number	US-11808697-B2
Application number	US-202217877680-A
Country	US
Kind code	B2
Filing date	Jul 29, 2022
Priority date	Oct 6, 2017
Publication date	Nov 7, 2023
Grant date	Nov 7, 2023

Abstract

Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

First claim

What is claimed is:

1. A carrier for holding a sample for analysis, the carrier comprising:
a porous membrane having a number of sample loading areas designated thereon, the porous membrane defining a plurality of openings extending through the membrane, wherein each of the sample loading areas is defined by at least some of the plurality of openings;
wherein at least the sample areas are impregnated with a surfactant.

2. The carrier of claim 1 , wherein the membrane comprises polyvinylidene difluoride, (PVDF), polytetrafluoroethylene (PTFE), or nitrocellulose.

3. The carrier of claim 1 , wherein the membrane comprises a woven mesh.

4. The carrier of claim 1 , wherein the sample loading area is hydrophobic.

5. The carrier of claim 1 , wherein the sample loading area is hydrophilic.

6. The carrier of claim 1 , wherein all of the carrier is impregnated with the surfactant.

7. The carrier of claim 1 , wherein the surfactant at least one of: shifts an absorbance spectra of the sample area; increases specific absorbance; decreases specific absorbance; and creates a new absorbance.

8. The carrier of claim 1 , wherein a bridge of the membrane extends between each adjacent pair of openings.

9. The carrier of claim 8 , wherein each of the sample loading areas is joined to a remainder of the membrane via the bridges between each adjacent pair of openings defining the sample loading area.

10. The carrier of claim 8 , wherein each of the bridges is configured to block migration of fluid through the bridge.

11. The carrier of claim 10 , wherein the bridges are at least one of: compressed to block migration of fluid; and treated with a substance to block migration of fluid.

12. The carrier of claim 11 , wherein the substance comprises at least one of: a solidifying substance; and an adhesive.

13. The carrier of claim 1 , wherein the carrier is transparent to infrared light.

14. The carrier of claim 1 , wherein the sample loading area is defined by at least one border marking.

15. The carrier of claim 14 , wherein the at least one border marking is hydrophobic.

16. The carrier of claim 15 , wherein the sample loading area is hydrophilic.

17. The carrier of claim 1 , wherein the sample loading area is defined by a compressed annular region of the carrier.

18. The carrier of claim 17 wherein the compressed annular region of the carrier surrounds the sample loading area.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Thrush Evan
• Swihart Steven
• Strong William
• Marlowe Trey
• Lu Li

Classifications

• G01N21/3103 Primary

  Atomic absorption analysis · CPC title

• G01N21/3563

  for analysing solids; Preparation of samples therefor · CPC title

• G01N21/3577

  for analysing liquids, e.g. polluted water · CPC title

• G01N2021/3572

  Preparation of samples, e.g. salt matrices · CPC title

• G01N2201/0686

  Cold filter; IR filter · CPC title