Protein quantitation device

Patent metadata

Field	Value
Publication number	US-11435285-B2
Application number	US-202117199312-A
Country	US
Kind code	B2
Filing date	Mar 11, 2021
Priority date	Oct 6, 2017
Publication date	Sep 6, 2022
Grant date	Sep 6, 2022

Abstract

Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

First claim

What is claimed is:

1. A device for measuring absorption of light by a sample, the device comprising:
a source of infrared radiation;
a carrier comprising a porous membrane, the carrier configured to hold a sample, wherein the porous membrane comprises a plurality of sample loading areas each designated by at least one border feature, wherein the at least one border feature comprises at least one of:
a plurality of border markings printed onto the porous membrane; and

compressed areas of the porous membrane, wherein the compressed areas prevent migration of sample out of the sample loading areas;
a sample holder positioned to place the sample on the carrier in a location to receive infrared radiation from the source;
a Fabry-Perot interferometer positioned to receive infrared radiation originating from the source and passing through the sample, the Fabry-Perot interferometer comprising a pair of spaced-apart reflective surfaces, at least one of which is movable to change the spacing between the reflective surfaces;
a detector positioned to receive infrared radiation from the Fabry-Perot interferometer and to produce an output signal indicating an intensity of the infrared radiation received at the detector; and
a controller coupled to the source, the Fabry-Perot interferometer, and the detector, the controller including a processor programmed to:
cause the Fabry-Perot interferometer to be tuned to a series of different resonant wavelengths;
receive the output signal of the detector at each of the series of resonant wavelengths; and
record an absorbance spectrum indicating the absorbance of the sample as a function of infrared radiation wavelength.

2. The device of claim 1 , wherein the at least one border features is hydrophobic.

3. The device of claim 1 , and wherein the porous membrane defines a number of openings through the porous membrane, each of the sample loading areas being surrounded by openings such that each of the sample loading areas is defined on a portion of the porous membrane joined to the remainder of the porous membrane by bridges of the porous membrane material.

4. The device of claim 3 , wherein the bridges are treated with a solidifying substance.

5. The device of claim 3 , wherein the solidifying substance comprises an adhesive.

6. The device of claim 1 , wherein at least one of:
the porous membrane material is polyvinylidene difluoride, (PVDF), polytetrafluoroethylene (PTFE), or nitrocellulose; and
the sample areas of the carrier are impregnated with a surfactant.

7. A system for measuring absorption of light by a sample, the system comprising:
a carrier comprising a porous membrane, the carrier configured to hold a sample, wherein the porous membrane comprises a plurality of sample loading areas each designated by at least one border feature, and wherein the at least one border feature comprises at least one of:
a plurality of border markings printed onto the porous membrane; and

compressed areas of the porous membrane, wherein the compressed areas prevent migration of sample out of the sample loading areas; and
a quantitation device comprising:
a source of infrared radiation;
a sample holder positioned to place a sample in a location to receive infrared radiation from the source, the sample holder configured to support the carrier;
a Fabry-Perot interferometer positioned to receive infrared radiation originating from the source and passing through the sample, the Fabry-Perot interferometer comprising a pair of spaced-apart reflective surfaces, at least one of which is movable to change the spacing between the reflective surfaces;
a detector positioned to receive infrared radiation from the Fabry-Perot interferometer and to produce an output signal indicating an intensity of the infrared radiation received at the detector; and
a controller coupled to the source, the Fabry-Perot interferometer, and the detector, the controller including a processor programmed to:
cause the Fabry-Perot interferometer to be tuned to a series of different resonant wavelengths;
receive the output signal of the detector at each of the series of resonant wavelengths; and
record an absorbance spectrum indicating the absorbance of the sample as a function of infrared radiation wavelength.

8. The system of claim 7 , wherein the at least one border features is hydrophobic.

9. The system of claim 7 , and wherein the porous membrane defines a number of openings through the porous membrane, each of the sample loading areas being surrounded by openings such that each of the sample loading areas is defined on a portion of the porous membrane joined to the remainder of the porous membrane by bridges of the porous membrane material.

10. The system of claim 9 , wherein the bridges are treated with a solidifying substance.

11. The system of claim 10 , wherein the solidifying substance comprises an adhesive.

12. The system of claim 7 , wherein the porous membrane material is polyvinylidene difluoride, (PVDF), polytetrafluoroethylene (PTFE), or nitrocellulose.

13. The system of claim 7 , wherein at least the sample areas of the carrier are impregnated with a surfactant.

14. The system of claim 7 , the quantitation device further comprising a band pass optical filter positioned in an optical path of the quantitation device such that infrared radiation from the source passes through the optical filter before reaching the detector.

15. The system of claim 14 , wherein the band pass optical filter is implemented using a coating on an optical element of the quantitation device.

16. The system of claim 14 , therein the band pass optical filter is a first band pass optical filter and passes infrared radiation in a first wavalength band, the quantitation device further comprising:
a second band pass optical filter that passes infrared radiation in a second wavelength band different from the first wavelength band; and
a mechanism for removing the first optical filter from the optical path and placing the second filter in the optical path such that infrared radiation from the source passes through the second optical filter beore reaching the detector.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Thrush Evan
• Swihart Steven
• Strong William
• Marlowe Trey
• Lu Li

Classifications

• G01N21/3103 Primary

  Atomic absorption analysis · CPC title

• G02B5/281

  designed for the infrared light · CPC title

• G01N2021/3572

  Preparation of samples, e.g. salt matrices · CPC title

• G01N21/3563

  for analysing solids; Preparation of samples therefor · CPC title

• G01J3/42 Primary

  Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry (beam switching arrangements G01J3/08) · CPC title