Method for extracting RG-I-rich pectin

Patent metadata

Field	Value
Publication number	US-11292852-B2
Application number	US-201716648227-A
Country	US
Kind code	B2
Filing date	Sep 26, 2017
Priority date	Sep 26, 2017
Publication date	Apr 5, 2022
Grant date	Apr 5, 2022

Abstract

The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature. The extracted pectic polysaccharide can be potentially used as functional food ingredients.

First claim

What is claimed is:

1. A method for extracting RG-I-rich pectin, comprising the following steps:
(1) drying and crushing citrus fruit peels, mixing the dried and crushed citrus fruit peels with 0.1 to 1.0 wt % of a dilute hydrochloric acid solution according to a solid-liquid ratio of 1:30 g/ml, performing magnetically stirring at a constant temperature of 10 to 40° C. for 10 to 120 min, and then performing filtering with a 400-mesh filter bag to obtain a filtrate and a retentate;
(2) dispersing the retentate obtained in step (1) in 0.1 to 2.0 wt % of a sodium hydroxide solution having an equal volume to that of the dilute hydrochloric acid used in step (1), performing magnetically stirring at a temperature of 10 to 40° C. for 5 to 120 min, and performing filtering with a 400-mesh filter bag;
(3) adjusting pH of a filtrate obtained in step (2) to 6 to 7 with 2 M hydrochloric acid, then performing precipitation with 95% by volume of ethanol having an equal volume to that of the filtrate obtained in step (2) for 2 to 3 h, and, performing filtering with a 400-mesh filter bag after the precipitation is completed to obtain a retentate alkali-extracted pectic polysaccharide; and
(4) washing the retentate alkali-extracted pectic polysaccharide 1 to 4 times with 95 wt % of ethanol to obtain a refined alkali-extracted pectic polysaccharide.

2. The method according to claim 1 , wherein a mass fraction of HCL in the dilute hydrochloric acid solution is 0.3% to 0.7%, and a mass fraction of NaOH in the sodium hydroxide solution is 0.3 to 0.7%.

3. The method according to claim 1 , wherein mixing and stirring of the dilute hydrochloric acid solution and the dried and crushed citrus fruit peels are controlled at a temperature of 20° C. to 40° C., and extraction with the sodium hydroxide solution in step (2) is controlled at a temperature of 20° C. to 40° C.

4. The method according to claim 1 , wherein stirring of the dilute hydrochloric acid solution and the dried and crushed citrus fruit peels is controlled within 30-60 min, and extraction with the sodium hydroxide solution in step (2) is controlled within 10-50 min.

5. The method according to claim 1 , wherein after the alkali-extracted pectic polysaccharide is obtained from alcohol precipitation, the polysaccharide is washed 2 to 3 times with 95% ethanol, so as to obtain a polysaccharide having good properties after drying.

Assignees

• Univ Zhejiang

Inventors

• Chen Shiguo
• Zhang Hua
• Ye Xingqian
• Liu Donghong
• Zheng Jiaqi

Classifications

• C08B37/0048 Primary

  Processes of extraction from organic materials · CPC title

• A23C9/13

  using additives · CPC title