Ultrasonic-assisted method of extracting a pectin rich in RG-I

Patent metadata

Field	Value
Publication number	US-11485799-B2
Application number	US-201917267489-A
Country	US
Kind code	B2
Filing date	Dec 3, 2019
Priority date	Dec 3, 2019
Publication date	Nov 1, 2022
Grant date	Nov 1, 2022

Abstract

The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

First claim

What is claimed is:

1. An ultrasonic-assisted method of extracting a pectin rich in RG-I, comprising:
(1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride;
(2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1);
(3) centrifuging the solution subjected to step (2) at a speed of 6000 to 8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and
(4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

2. The ultrasonic-assisted method of extracting a pectin rich in RG-I according to claim 1 , wherein, in step (1), the citrus peel powders are mixed with the alkaline solution according to a solid-liquid ratio of 1:50 g/mL, and an alkali in the alkaline solution is sodium hydroxide or potassium hydroxide with a concentration of 0.05 to 0.1 mol/L, and a concentration of sodium borohydride is 20 to 50 mmol/L.

3. The ultrasonic-assisted method of extracting a pectin rich in RG-1 according to claim 1 , wherein in step (2), an ultrasonic time is 20 to 40 min, and an ultrasonic intensity is 0.5 to 3.5 W/mL.

4. The ultrasonic-assisted method of extracting a pectin rich in RG-I according to claim 1 , wherein a centrifuging time in step (3) is 20 to 30 min.

5. The ultrasonic-assisted method of extracting a pectin rich in RG-I according to claim 1 , wherein the pectic polysaccharide precipitant in step (4) is washed with absolute ethanol for 2 to 3 times.

Assignees

• Univ Zhejiang

Inventors

• Ye Xingqian
• Hu Weiwei
• Chen Shiguo
• Cheng Huan
• Liu Donghong

Classifications

• C08B37/0045 Primary

  alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof · CPC title

• C08B37/0048 Primary

  Processes of extraction from organic materials · CPC title