Hydrophilic polymeric particles and methods for making and using same

Patent metadata

Field	Value
Publication number	US-10947333-B2
Application number	US-201916259963-A
Country	US
Kind code	B2
Filing date	Jan 28, 2019
Priority date	Feb 9, 2012
Publication date	Mar 16, 2021
Grant date	Mar 16, 2021

Abstract

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

First claim

What is claimed is:

1. A method for forming a polymer, comprising:
in a water-immiscible phase, providing mer units of a hydrophilic acrylamide monomer having a hydrophobic protection group, wherein the hydrophilic acrylamide monomer includes hydroxyl groups, amino groups, carboxyl groups, or a combination thereof;
forming a polymeric particle having a plurality of the hydrophobic protection group;
cleaving the hydrophobic protection groups from the polymeric particle to form a hydrophilic polymeric particle;
activating the hydrophilic polymer particle; and
conjugating a nucleic acid to the activated hydrophilic polymeric particle.

2. The method of claim 1 , wherein the hydrophobic protection group is silyl.

3. The method of claim 1 , wherein the hydrophobic protection group is alkyloxycarbonyl group.

4. The method of claim 1 , wherein the hydrophobic protection group is alkoxy group.

5. The method of claim 1 , wherein the acrylamide monomer is (N-(2-((tert-butyldimethylsilyl)oxy)ethyl)acrylamide), (N-(2-((triethylsilyl)oxy)ethyl)acrylamide), or combinations thereof.

6. The method of claim 1 , wherein the water-immiscible phase further includes a crosslinker.

7. The method of claim 1 , wherein the water-immiscible phase is a disperse phase within an aqueous suspension.

8. The method of claim 7 , wherein forming the polymeric particle comprises polymerizing the mer units in the disperse phase.

9. The method of claim 8 , further comprising promoting a seed particle in the aqueous suspension to form the disperse phase.

10. The method of claim 9 , further comprising adding a porogen to the disperse phase.

11. The method of claim 9 , wherein the seed particle comprises a seed polymer that comprises a styrenic polymer, an acrylic polymer, an acrylamide, another hydrophobic vinyl polymer, or any combination thereof.

12. The method of claim 11 , wherein the disperse phase is a hydrophobic phase and comprises, at least at the beginning of the polymerization, droplets of mer units from which the mer units diffuse through the aqueous phase of the emulsion to the seed particles, a surfactant aiding the diffusion.

13. The method of claim 12 , wherein forming the polymeric particle comprises heating the emulsion of mer units and starting particle nucleation in the aqueous phase of the emulsion by adding a water-soluble initiator.

Assignees

• Life Technologies Corp
• Life Tech As

Inventors

• Fonnum Geir
• Modahl Grete Irene
• Kjus Nini Hofsloekken
• Molteberg Astrid Evenroed
• Tran Diem Thuy Thi
• Aaserud Jo
• Gokmen Muhammed
• Menchen Steven M
• Fuller Carl
• Andruzzi Luisa
• Hinz Wolfgang

Classifications

• C09D5/00

  Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced {(electrically insulating plastics, resins or waxes H01B3/30)}; Filling pastes · CPC title

• C08J3/075

  Macromolecular gels · CPC title

• C08F257/02

  on to polymers of styrene or alkyl-substituted styrenes · CPC title

• C08F2/16 Primary

  Aqueous medium · CPC title

• C08F230/08

  containing silicon · CPC title