Hydrophilic polymeric particles and methods for making and using same

Patent metadata

Field	Value
Publication number	US-9243085-B2
Application number	US-201313762941-A
Country	US
Kind code	B2
Filing date	Feb 8, 2013
Priority date	Feb 9, 2012
Publication date	Jan 26, 2016
Grant date	Jan 26, 2016

Abstract

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

First claim

What is claimed is:

1. A method of forming a particle, the method comprising:
promoting a seed particle in an aqueous suspension to form a disperse phase within the aqueous suspension, wherein promoting the seed particle includes mixing a solvent and a promoting agent with the seed particle;
adding a hydrophilic monomer having a hydrophobic protection group to the disperse phase;
in the disperse phase, polymerizing a plurality of mer units of the hydrophilic monomer, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups; and
converting the polymeric particle to a hydrogel particle.

2. The method of claim 1 , wherein converting the polymeric particle includes removing at least a portion of the plurality of the hydrophobic protection groups from the polymeric particle.

3. The method of claim 2 , wherein removing at least a portion of the plurality of the hydrophobic protection groups includes acid cleaving at least a portion of the plurality of the hydrophobic protection groups from the polymeric particle.

4. The method of claim 1 , wherein the seed particle includes a seed polymer, the method further comprising extracting the seed polymer after converting the polymeric particle.

5. The method of claim 4 , wherein the seed polymer is hydrophobic.

6. The method of claim 1 , wherein the hydrophilic monomer includes an acrylamide.

7. The method of claim 1 , wherein the hydrophobic protection group includes an organometallic moiety.

8. The method of claim 7 , wherein the organometallic moiety forms a silyl ether functional group.

9. The method of claim 1 , wherein polymerizing the plurality of mer units further includes mixing a crosslinker with the hydrophilic monomer having a hydrophobic protection group.

10. The method of claim 9 , wherein the crosslinker is a divinyl crosslinker.

11. The method of claim 1 , further comprising activating the hydrogel particle.

12. The method of claim 11 , further comprising binding an oligonucleotide to the activated hydrogel polymer.

13. The method of claim 12 , wherein binding includes nucleophilic substitution and the oligonucleotide is a nucleophile-terminated oligonucleotide.

14. The method of claim 12 , further comprising hybridizing a polynucleotide to the oligonucleotide.

15. The method of claim 14 , further comprising amplifying the polynucleotide into a plurality of complementary polynucleotides by extending the oligonucleotide, thereby generating a hydrogel particle including a plurality of attached polynucleotides.

16. A method of forming a particle, the method comprising:
promoting a seed particle in an aqueous suspension to form a disperse phase within the aqueous suspension, wherein promoting the seed particle includes mixing a solvent and a promoting agent with the seed particle;
adding an acrylamide monomer having a hydrophobic protection group to the disperse phase;
in the disperse phase, polymerizing a plurality of mer units of the acrylamide monomer, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups; and
converting the polymeric particle to a hydrophilic particle.

17. A method of forming a particle, the method comprising:
promoting a seed particle in an aqueous suspension to form a disperse phase within the aqueous suspension, wherein promoting the seed particle includes mixing a solvent and a promoting agent with the seed particle;
adding a radically polymerizable monomer and the dyacrylamide crosslinker having a hydrophobic protection group;
in the disperse phase, polymerizing a plurality of mer units of the radically polymerizable monomer with the diacrylamide crosslinker, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups; and
removing at least a portion of plurality of the hydrophobic protection groups.

18. The method of claim 17 , wherein the diacrylamide includes N,N′-(ethane-1,2-diyl)bis(N-(2-((tert-butyldimethylsilyl)oxy)ethyl)acrylamide, N,N′-(2-hydroxypropane-1,3-diyl)diacrylamide, a protected derivative thereof, or a combination thereof.

19. The method of claim 6 , wherein the acrylamide is an acrylamide including hydroxyl groups, amino groups, carboxyl groups, or a combination thereof.

Assignees

• Life Technologies Corp
• Life Technologies As

Inventors

• Fonnum Geir
• Modahl Grete
• Kjus Nini
• Molteberg Astrid Evenroed
• Tran Diem
• Aaserud Jo
• Gokmen M Talha
• Menchen Steven
• Fuller Carl
• Andruzzi Luisa
• Hinz Wolfgang

Classifications

• C07F7/188

  by reactions involving the formation of Si-O linkages · CPC title

• C08F222/385

  Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide · CPC title

• C08F2/20

  with the aid of macromolecular dispersing agents · CPC title

• G01N27/4145

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• C08J2343/04

  Homopolymers or copolymers of monomers containing silicon · CPC title