Polymer substrates formed from carboxy functional acrylamide

Patent metadata

Field	Value
Publication number	US-9868826-B2
Application number	US-201615200533-A
Country	US
Kind code	B2
Filing date	Jul 1, 2016
Priority date	Jul 2, 2015
Publication date	Jan 16, 2018
Grant date	Jan 16, 2018

Abstract

A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

First claim

What is claimed is:

1. A method of forming a particle, the method comprising:
in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units having the formula:

wherein R 1 is an alkyl group having between 3 and 10 carbons or is a polyether group having between 1 and 10 ether units, wherein R 2 is a linear or branched alkyl group having between 3 and 8 carbons or a silyl group, and wherein R 3 is hydrogen or an alkyl group having between 1 and 6 carbons;
thereby forming a polymeric particle including a plurality of hydrophobic protection groups; and
converting the polymeric particle to a hydrophilic particle.

2. The method of claim 1 , wherein the hydrophilic particle is a hydrogel particle.

3. The method of claim 1 , further comprising a hydrophilic monomer in the dispersed phase, wherein the hydrophilic monomer includes an acrylamide monomer.

4. The method of claim 1 , wherein the dispersed phase further includes a diacrylamide crosslinker having a hydrophobic protection group.

5. The method of claim 1 , wherein converting the polymeric particle includes removing at least a portion of the plurality of the R 2 groups from the polymeric particle.

6. The method of claim 5 , wherein removing at least a portion of the plurality of the R 2 groups includes acid cleaving at least a portion of the plurality of the R 2 groups from the polymeric particle.

7. The method of claim 1 , further comprising promoting a seed particle in the aqueous suspension to form the dispersed phase.

8. The method of claim 7 , wherein the seed particle includes a seed polymer.

9. The method of claim 8 , further comprising extracting the seed polymer after converting the polymeric particle.

10. The method of claim 8 , wherein the seed polymer is hydrophobic.

11. The method of claim 8 , wherein the seed polymer includes a styrenic polymer, an acrylic polymer, an acrylamide, another vinyl polymer, or a combination thereof.

12. The method of claim 7 , wherein promoting the seed particle includes mixing a solvent and a promoting agent with the seed particle.

13. The method of claim 12 , wherein the promoting agent includes dioctanoyl peroxide or dioctyladipate or polystyrene with molecular weight below 20 kD.

14. The method of claim 1 , wherein the dispersed phase further includes acrylamide, hydroxyalkyl acrylamide, or a combination thereof, the acrylamide or hydroxyalkyl acrylamide polymerizing with the plurality of mer units.

15. The method of claim 1 , wherein polymerizing the plurality of mer units further includes mixing a crosslinker with the plurality of mer units.

16. The method of claim 15 , wherein the crosslinker is a divinyl crosslinker.

17. The method of claim 16 , wherein the divinyl crosslinker includes a diacrylamide.

18. The method of claim 1 , wherein polymerizing the plurality of mer units includes mixing a porogen in the disperse phase.

19. The method of claim 1 , further comprising activating the hydrophilic particle, wherein activating includes applying a succinimidyl compound to the hydrophilic particle.

20. The method of claim 19 , further comprising binding an oligonucleotide to the activated hydrogel polymer.

Assignees

• Life Technologies Corp
• Life Tech As

Inventors

• Fonnum Geir
• Menchen Steven M
• Gokmen Talha M
• Lundberg Pontus
• Thwar Prasanna Krishnan
• Lui Alfred
• Lu Lily
• Hinz Wolfgang
• Husaboe Lene
• Breivold Elisabeth
• Molteberg Astrid Evenroed
• Larsen Synne

Classifications

• C08F230/08

  containing silicon · CPC title

• C08J2333/26

  Homopolymers or copolymers of acrylamide or methacrylamide · CPC title

• C08F220/70

  Nitriles; Amides; Imides · CPC title

• C08F8/12

  Hydrolysis · CPC title

• C08J3/075 Primary

  Macromolecular gels · CPC title