Method for fermentative production of oxidized coenzyme Q10

Patent metadata

Field	Value
Publication number	US-10774350-B2
Application number	US-201816016813-A
Country	US
Kind code	B2
Filing date	Jun 25, 2018
Priority date	Dec 25, 2017
Publication date	Sep 15, 2020
Grant date	Sep 15, 2020

Abstract

The present application relates to a method for fermentative production of oxidized coenzyme Q10 and high-content oxidized coenzyme Q10 prepared therefrom. For the method for fermentative production of oxidized coenzyme Q10, in a fermentation process of a production strain, the oxidation-reduction potential (ORP) of a fermentation broth is controlled to be −50 to 300 Mv, and preferably the oxidation-reduction potential (ORP) of the fermentation broth is controlled to be 50 to 200 mV. By controlling the ORP of the fermentation broth, the method for fermentative production of oxidized coenzyme Q10 enables the oxidized coenzyme Q10 content in the coenzyme Q10 produced by microorganisms to reach 96% or more, and the product is substantially composed of a single component, which makes post-treatment more convenient. Oxidized coenzyme Q10 is more stable than reduced coenzyme Q10, and as compared with the coenzyme Q10 obtained by fermentative production in the prior art, high-content oxidized coenzyme Q10 degrades in a less amount in organisms. In addition, the fermentation method of the present application has a high potency.

First claim

What is claimed is:

1. A method for fermentative production of oxidized coenzyme Q10 in a fermenter, which comprises:
controlling the oxidation-reduction potential (ORP) of a fermentation broth containing a member selected from the group consisting of Rhodobacter sphaeroides strain of Deposit No. CGMCC No. 5997, Rhodobacter sphaeroides strain of Deposit No. CGMCC No. 5998, or Rhodobacter sphaeroides strain of Deposit No. CGMCC No. 5999 to be between −50 to −300 mV by at least one of controlling the stirring input power per unit volume of the fermenter to be between 0.25 and 0.50 kw/m 3 , controlling the air inlet flow per unit volume of the fermentation broth in the fermenter to be between 1.0 and 15.0 vvm and controlling the internal pressure of the fermenter to be between 0.05 and 0.3 MPa, and
maintaining the conductivity of the fermentation broth between 5.0 and 30.0 ms/cm by adding to the broth a feed solution having a pH of 7.0 and containing 0.013 to 0.025 grams of biotin per liter of feed solution.

2. The method for fermentative production according to claim 1 wherein the oxidation-reduction potential (ORP) of the fermentation broth is controlled by at least one of the following means: adjusting the dissolved oxygen of said fermentation broth, and controlling the pH of said fermentation broth.

3. The method for fermentative production according to claim 2 , wherein the pH of said fermentation broth is controlled by controlling the pH of said fermentation broth to be 3.5 to 6.0.

4. The method for fermentative production according to claim 1 , wherein the ORP of the fermentation broth is controlled in a coenzyme Q10 synthesis and accumulation stage in the fermentation process.

5. The method for fermentative production according to claim 1 , wherein said coenzyme Q10 is high-content oxidized coenzyme Q10.

6. The method for fermentative production according to claim 1 , wherein in a fermentation process of a production strain, the oxidation-reduction potential (ORP) of a fermentation broth is controlled to be 50 to 200 mV.

7. The method for fermentative production according to claim 1 , wherein said stirring input power per unit volume of the fermenter is 0.30 to 0.40 kw/m 3 , said air inlet flow per unit volume of the fermentation broth is 5.0 to 8.0 vvm, and/or said internal pressure of the fermenter is 0.08 to 0.15 MPa.

8. The method for fermentative production according to claim 3 , wherein the pH of said fermentation broth is controlled by controlling the pH of said fermentation broth to be 4.0 to 5.0.

9. The method for fermentative production according to claim 3 , wherein the pH of said fermentation broth is controlled by means of adding an acid or a base in phases or continuously.

10. The method for fermentative production according to claim 9 , wherein said acid is one or two or more of phosphoric acid, hydrochloric acid, sulfuric acid, lactic acid, propionic acid, citric acid, and oxalic acid, and/or said base is one or two or more of ammonia water, sodium hydroxide, and liquid ammonia.

11. The method for fermentative production according to claim 10 , wherein said acid is phosphoric acid, lactic acid, or citric acid, and/or said base is ammonia water or liquid ammonia.

12. The method for fermentative production according to claim 4 , wherein the ORP of the fermentation broth is controlled in a middle or a late phase of the coenzyme Q10 synthesis and accumulation stage in the fermentation process.

13. The method for fermentative production according to claim 1 , wherein in a bacterial growth stage, the oxygen consumption rate is controlled to be between 30 and 150 mmol/(L·h) and the conductivity of said fermentation broth is controlled to be between 5.0 and 30.0 ms/cm,
and/or, in a coenzyme Q10 synthesis and accumulation stage, the oxygen consumption rate is controlled to be between 60 and 120 mmol/(L·h), and the conductivity of said fermentation broth is controlled between 8.0 and 15.0 ms/cm.

Assignees

• Zhejiang Nhu Co Ltd
• Univ Zhejiang
• Heilongjiang Nhu Biotechnology Company Ltd
• Shangyu Nhu Biological Chemical Co Ltd

Inventors

• Yuan Shenfeng
• Yu Hongwei
• Chen Zhaofeng
• Zhu Yongqiang
• Min Yi
• Li Yong
• Hu Baishan
• Qiu Guisheng
• Yu Kai

Classifications

• C12P7/66 Primary

  containing the quinoid structure · CPC title