Polypeptides having peroxygenase activity

Patent metadata

Field	Value
Publication number	US-9920341-B2
Application number	US-201715454544-A
Country	US
Kind code	B2
Filing date	Mar 9, 2017
Priority date	Oct 12, 2012
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted, linear or branched, aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or 3, comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity, wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

2. A method for hydroxylation in position 2 or 3 of the terminal end of an acyl group of a lipid, comprising contacting the lipid with hydrogen peroxide and a polypeptide having peroxygenase activity, wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

3. A method for introducing a hydroxy or a keto group at the second or third carbon of at least two ends of a substituted or unsubstituted, linear or branched, aliphatic hydrocarbon having at least five carbons and having at least one hydrogen attached to said second or third carbon, comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity, wherein the polypeptide has at least 80% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

4. The method of claim 1 , wherein the aliphatic hydrocarbon is an alkane.

5. The method of claim 2 , wherein the aliphatic hydrocarbon is an alkane.

6. The method of claim 3 , wherein the aliphatic hydrocarbon is an alkane.

7. The method of claim 4 , wherein the alkane is pentane, hexane, heptane, octane, nonane, decane, undecane, dodecane, tridecane, tetradecane, pentadecane or hexadecane, or an isomer thereof.

8. The method of claim 5 , wherein the alkane is pentane, hexane, heptane, octane, nonane, decane, undecane, dodecane, tridecane, tetradecane, pentadecane or hexadecane, or an isomer thereof.

9. The method of claim 6 , wherein the alkane is pentane, hexane, heptane, octane, nonane, decane, undecane, dodecane, tridecane, tetradecane, pentadecane or hexadecane, or an isomer thereof.

10. The method of claim 1 , wherein the aliphatic hydrocarbon is a fatty acid.

11. The method of claim 2 , wherein the aliphatic hydrocarbon is a fatty acid.

12. The method of claim 3 , wherein the aliphatic hydrocarbon is a fatty acid.

13. The method of claim 1 , wherein the polypeptide has at least 85% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

14. The method of claim 1 , wherein the polypeptide has at least 90% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

15. The method of claim 1 , wherein the polypeptide has at least 95% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

16. The method of claim 1 , wherein the polypeptide has at least 97% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

17. The method of claim 2 , wherein the polypeptide has at least 85% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

18. The method of claim 3 , wherein the polypeptide has at least 90% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

19. The method of claim 4 , wherein the polypeptide has at least 95% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

20. The method of claim 5 , wherein the polypeptide has at least 97% sequence identity to the polypeptide of amino acids 1-241 of SEQ ID NO: 2.

Assignees

• Novozymes As

Inventors

• Landvik Sara
• Oestergaard Lars Henrik
• Kalum Lisbeth

Classifications

• C12N9/0065 Primary

  acting on hydrogen peroxide as acceptor (1.11) · CPC title

• C12P7/00

  Preparation of oxygen-containing organic compounds · CPC title

• C12P7/18

  polyhydric · CPC title

• C12P7/24

  containing a carbonyl group · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title