Vanillin Synthase
US-2015267227-A1 · Sep 24, 2015 · US
Glycosyltransferase glycosylating flavokermesic acid and/or or kermesic acid
US10100290B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10100290-B2 |
| Application number | US-201415105673-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 18, 2014 |
| Priority date | Dec 18, 2013 |
| Publication date | Oct 16, 2018 |
| Grant date | Oct 16, 2018 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid.
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant expression vector comprising a nucleic acid construct comprising an isolated polynucleotide comprising a nucleotide sequence which encodes an isolated glycosyltransferase polypeptide capable of: (I): conjugating nucleotide activated glucose to flavokermesic acid (FK); and/or (II): conjugating nucleotide activated glucose to kermesic acid (KA); and wherein the glycosyltransferase polypeptide is at least one polypeptide selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence which has at least 95% identity with amino acids 1 to 515 of SEQ ID NO:2; and (b) a polypeptide comprising an amino acid sequence which has at least 95% identity with amino acids 20 to 468 of SEQ ID NO:2; and wherein said polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 2. A recombinant host cell comprising the recombinant expression vector of claim 1 . 3. The recombinant host cell of claim 2 , wherein the recombinant host cell is a cell selected from the group consisting of a filamentous fungal cell and a microorganism cell, and wherein if the microorganism cell is a yeast cell, the yeast cell is selected from the group consisting of Saccharomyces spp, and Pichia spp. 4. The recombinant host cell of claim 3 , wherein the recombinant host cell is Saccharomyces cerevisiae.
Assignees
Inventors
Classifications
Glycosyltransferases (2.4) · CPC title
for yeasts · CPC title
Hexosyltransferases (2.4.1) · CPC title
- C12N9/1048Primary
Glycosyltransferases (2.4) · CPC title
Bacteria; Culture media therefor · CPC title
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Frequently asked questions
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- What does patent US10100290B2 cover?
- An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid.
- Who is the assignee on this patent?
- Univ Copenhagen, Univ Danmarks Tekniske
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1048. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 16 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).