Viscosity measurements based on tracer diffusion

Patent metadata

Field	Value
Publication number	US-10620102-B2
Application number	US-201615741333-A
Country	US
Kind code	B2
Filing date	Jun 29, 2016
Priority date	Jul 2, 2015
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

A method is provided for measuring the viscosity of a fluid sample. The method comprising the steps of: (ii) providing a flow of the fluid sample; (iii) providing a component flow, wherein the component flow is a flow of the fluid sample further comprising a tracer component; (iv) generating a laminar flow of the flow (ii) with the flow (iii) in a diffusion channel, such as a microfluidic diffusion channel (2); (iv) measuring the lateral diffusion of the tracer component across the flows; and (v) determining the viscosity of the fluid from the measured diffusion profile, wherein the size of the tracer component is known or is determined.

First claim

The invention claimed is:

1. A method for measuring the viscosity of a fluid sample, the method comprising the steps of:
(i) providing a first flow of the fluid sample;
(ii) providing a component flow, wherein the component flow is a second flow of the fluid sample further comprising a tracer component;
(iii) generating a laminar flow of the first flow with the second flow in a diffusion channel, such as a microfluidic diffusion channel;
(iv) measuring the lateral diffusion of the tracer component across the flows; and
(v) determining the viscosity of the fluid from the measured diffusion profile, wherein the size of the tracer component is known or is determined.

2. The method of claim 1 , comprising the preliminary step of adding a tracer component to a part of the fluid sample.

3. The method of claim 1 , wherein step (iv) is measuring the lateral diffusion of the tracer component across the flows at a plurality of diffusion times.

4. The method of claim 1 , wherein the tracer component is fluorescent, and the lateral diffusion of the component is measured by fluorescence.

5. The method of claim 1 , wherein the channel is a microfluidic channel.

6. The method of claim 1 , wherein the first flow and the second flow are brought into contact in a large cross section channel, and the contacting flows are permitted to flow from the large cross section channel into the diffusion channel.

7. The method of claim 1 , wherein step (i) further comprises providing a third flow of the fluid sample, and the first and third fluid flows are brought into contact with and provided on opposite sides of the fluid flow comprising the tracer component.

8. The method of claim 1 , wherein the component has a radius, such as a hydrodynamic radius, in the range 0.5 to 200 nm, such as 0.5 to 100 nm.

9. The method of claim 1 , wherein the component flow is a flow of the fluid sample further comprising a tracer component, and the tracer component is present at 0.2 wt % or less.

10. The method of claim 1 , wherein the component is substantially monodisperse.

11. The method of claim 1 , wherein the component is a polymeric molecule.

12. The method of claim 1 , wherein the fluid sample is an aqueous sample.

13. The method of claim 1 , wherein the fluid sample comprises one or more components.

14. The method of claim 13 , wherein the fluid sample comprises a component having a polypeptide, polynucleotide or polysaccharide group.

15. The method of claim 13 , wherein the fluid sample comprises a component having a radius, such as a hydrodynamic radius, in the range 0.5 to 200 nm, such as 0.5 to 100 nm.

16. The method of claim 13 , wherein the tracer component has a radius, such as a hydrodynamic radius, that is from 10% to 200% that of the radius of a component in the fluid sample.

Assignees

• Cambridge Entpr Ltd

Inventors

• Mueller Thomas
• Knowles Tuomas
• Arosio Paolo

Classifications

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L3/502776

  specially adapted for focusing or laminating flows · CPC title

• B01L2400/0472

  Diffusion · CPC title

• G01N2013/003

  Diffusion; diffusivity between liquids · CPC title

• B01L2300/0883

  Serpentine channels · CPC title