Fluidic analysis and separation

Patent metadata

Field	Value
Publication number	US-10386332-B2
Application number	US-201515307221-A
Country	US
Kind code	B2
Filing date	Apr 30, 2015
Priority date	Apr 30, 2014
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

A method for analyzing a component is provided. The method includes the steps of: (iii) providing the electrophoretic or thermophoretic movement of the component into a second fluid flow; (iv) diverting a part of a first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part is a third fluid flow which includes, the component; (v) contacting the third fluid flow with a fourth fluid flow, such as to form a laminar flow; (vi) providing the diffusion of the component into the fourth fluid flows.

First claim

The invention claimed is:

1. A method for analyzing a component, the method comprising the steps of:
(i) providing the component in a first fluid flow;
(ii) contacting the first fluid flow with a second fluid flow to generate a laminar flow,
(iii) providing electrophoretic or thermophoretic movement of the component into the second fluid flow;
(iv) diverting a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, wherein the diverted part is a third fluid flow which comprises the component;
(v) contacting the third fluid flow with a fourth fluid flow to generate a laminar flow;
(vi) providing diffusion of the component into the fourth fluid flow;
(vii) quantitatively determining a value of a first chemical or physical property of the component based on the electrophoretic or thermophoretic movement of the component into the second fluid flow; and
(viii) quantitatively determining a value of a second chemical or physical property of the component based on the diffusion of the component into the fourth fluid flow.

2. The method according to claim 1 , further comprising the step of:
(ix) diverting a part of the third fluid flow, a part of the fourth fluid flow, or parts of the third fluid flow and fourth fluid flow, wherein the diverted part is a fifth fluid flow which comprises the component.

3. The method of claim 2 , further comprising the subsequent steps of:
(xi) contacting the fifth fluid flow with a sixth fluid flow to form a laminar flow; and
(xii) providing a distribution of the component across contacting fifth and sixth fluid flows.

4. The method of claim 3 , further comprising the subsequent step of:
(xiii) diverting a part of the fifth fluid flow, a part of the sixth fluid flow, or parts of the fifth fluid flow and sixth fluid flow; wherein the diverted part is a seventh fluid flow which comprises the component.

5. The method according to claim 2 , further comprising the step of:
(x) subsequently labeling the component in the third fluid flow or the fifth fluid flow.

6. The method of claim 1 , wherein step (iii) comprises the electrophoretic movement of the component into the second fluid flow.

7. The method according to claim 6 , wherein the electrophoretic movement comprises isoelectric focusing.

8. The method according to claim 1 , wherein the component has a hydrodynamic radius in the range of 0.5 nm to 200 nm.

9. The method according to claim 1 , wherein the component has a hydrodynamic radius in the range of 0.5 nm to 25 nm.

10. The method according to claim 1 , wherein the second chemical or physical property of the component is a diffusion coefficient of the component.

11. The method according to claim 1 , wherein the second chemical or physical property of the component is a hydrodynamic radius of the component.

12. The method according to claim 1 , wherein the first chemical or physical property of the component is a charge-to-size ratio of the component.

13. The method according to claim 1 , wherein the first chemical or physical property of the component is an isoelectric point of the component.

14. The method according to claim 1 , wherein the first chemical or physical property of the component is an ionic mobility of the component.

15. The method according to claim 1 , wherein the first chemical or physical property of the component is the charge of the component.

16. The method according to claim 1 , wherein the second chemical or physical property of the component the property of the component is the mass of the component.

17. The method according to claim 1 , wherein quantitatively determining the value of the first chemical or physical property of the component and/or the quantitatively determining the value of the second chemical or physical property of the component comprises fitting an experimental diffusion profile of the component to a linear combination of basis functions.

18. The method according to claim 1 , wherein the first fluid further comprises a second component having a value of the first chemical or physical property different from the value of the first chemical or physical property of the first component, and further comprising determining the value of the first chemical or physical property of the second component.

19. The method according to claim 18 , wherein the second component has a value of the second chemical or physical property different from the value of the second chemical or physical property of the first component, and further comprising determining the value of the second chemical or physical property of the second component.

20. A flow apparatus for analyzing a component, the apparatus comprising:
a first separation channel for first and second flows in contact, wherein the separation channel is adapted to permit lateral movement of components between contacting first and second flows;
a first flow separator, in fluid communication with and downstream of the first separation channel, the first flow separator being adapted to divert a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, from the first separation channel, the diverted flow being a third flow;
a second separation channel, in fluid communication with and downstream of the first flow separator, the second separation channel being for third and fourth flows in contact, wherein the separation channel is adapted to permit lateral movement of components between contacting third and fourth flows;
wherein the apparatus further comprises a second flow separator, in fluid communication with and downstream of the second separation channel, the second flow separator being adapted to divert a part of the third fluid flow, a part of the fourth fluid flow, or parts of the third fluid flow and the fourth fluid flow, from the second separation channel, the diverted flow being a fifth flow; and
a reagent flow channel for introducing a reagent to a component in the fifth diverted flow, wherein the reagent is a label or is capable of generating a detectable label upon reaction,
wherein the flow apparatus is configured for quantitatively determining a value of a first chemical or physical property of the component based on the electrophoretic or thermophoretic movement of the component into the second fluid flow, and
wherein the flow apparatus is configured for quantitatively determining a value of a second chemical or physical property of the component based on the diffusion of the component into the fourth fluid flow.

21. The flow apparatus of claim 20 , wherein the apparatus further comprises a third separation channel, in fluid communication with and downstream of the second flow separator, the third separation channel being for fifth and sixth flows in contact, wherein the separation channel is adapted to permit lateral movement of components between contacting fifth and sixth flows.

22. The flow apparatus of claim 20 , wherein the apparatus further comprises an analysis zone which is downstream of and in fluid communication with a flow separator.

23. The method according to claim 22 , wherein the flow separator upstream of and in fluidic communication with the analysis zone is the second flow separator.

24. The flow apparatus according to claim 20 , wherein at least one of the first and second separation channels is provided with electrodes along the channel length.

Assignees

• Cambridge Entpr Ltd

Inventors

• Herling Therese
• Mueller Thomas
• Knowles Tuomas

Classifications

• B01L2400/0451

  Thermophoresis; Thermodiffusion; Soret-effect · CPC title

• B01L2400/0472

  Diffusion · CPC title

• B01L3/502776 Primary

  specially adapted for focusing or laminating flows · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title