Fluidic separation and detection

Patent metadata

Field	Value
Publication number	US-10295545-B2
Application number	US-201815922082-A
Country	US
Kind code	B2
Filing date	Mar 15, 2018
Priority date	Nov 14, 2013
Publication date	May 21, 2019
Grant date	May 21, 2019

Abstract

Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.

First claim

The invention claimed is:

1. A flow apparatus for detecting a component in a mixture, the apparatus comprising:
a separation channel for first and second flows in contact, wherein the separation channel is configured to permit lateral movement of components between contacting first and second flows;
a downstream flow separator in fluid communication with the separation channel, wherein the flow separator is configured to divert a part of the first fluid flow, a part of the second fluid flow, or parts of the first fluid flow and the second fluid flow, from the separation channel, and
a detection zone comprising:
a detection channel provided downstream of and in fluid communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator;
a label channel configured to subsequently label the component in the diverted flows from the flow separator;
an observation zone configured to record an analytical signal indicative of the quantity and the electrical properties of the component; and
a waste channel at the downstream end of the observation zone.

2. The flow apparatus according to claim 1 , further comprising two or more microfluidic channels for introducing at least first and second flows into the separation channel.

3. The flow apparatus according to claim 2 , wherein three microfluidic channels are provided.

4. The flow apparatus according to claim 1 , further comprising a reservoir for collecting the flow output from the observation zone and the non-diverted flow from the separation channel.

5. The flow apparatus according to claim 1 , wherein the waste channel is at the downstream end of the separation channel.

6. A method of analyzing a component, the method comprising the steps of:
providing the component in a first fluid flow;
contacting the fluid flow with a second fluid flow, such as to generate a laminar flow;
permitting the component to join the second fluid flow, thereby to obtain a distribution of the component across the first and second fluid flows;
applying an electric field to divert a plurality of parts of the first fluid flow and the second fluid flow by electrophoresis, wherein the diverted part comprises the component in its native state; and
analyzing the component in the diverted part of the fluid flow to obtain an analytical signal indicative of the quantity and the electrical properties of the component.

7. The method according to claim 6 , further comprising the step of introducing at least first and second flows into the separation channel.

8. The method according to claim 7 , wherein three flows are introduced into the separation channel.

9. The method according to claim 8 , wherein the three flows comprise the component flanked by sheathes of buffer.

10. The method according to claim 6 , wherein the first and second fluid flows are aqueous flows.

11. The method according to claim 6 , wherein the component is a biomolecule.

12. The method according to claim 11 , wherein the biomolecule is a protein.

13. The method according to claim 6 , wherein the distribution of the component across the first and second fluid flows is non-uniform.

14. The method according to claim 6 , further comprising labeling the component in the diverted part of the fluid flow.

15. The method according to claim 14 , wherein labelling the component comprises bonding a fluorescent label to the component.

16. The flow apparatus according to claim 1 , wherein the separation channel has a rectangular cross-section.

17. The flow apparatus according to claim 1 , wherein a surface of the separation channel is configured to limit or prevent absorption of the component thereon.

18. The flow apparatus according to claim 1 , wherein the label channel is configured to label the component by bonding a fluorescent label to the component.

19. The flow apparatus according to claim 1 , wherein the flow apparatus is configured to apply an electric field across the separation channel.

20. The flow apparatus according to claim 19 , wherein the electric field causes the lateral movement of the component.

Assignees

• Cambridge Entpr Ltd

Inventors

• Yates Emma
• Dobson Christopher
• Herling Therese
• Knowles Tuomas

Classifications

• B01L2300/0883

  Serpentine channels · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• G01N27/447

  using electrophoresis · CPC title

• G01N33/52

  Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper {and including single- and multilayer analytical elements (immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72)} · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title