Carrier including ammonium oxidizing bacteria immobilized therein and method for preparing same

Patent metadata

Field	Value
Publication number	US-10081804-B2
Application number	US-201615361539-A
Country	US
Kind code	B2
Filing date	Nov 28, 2016
Priority date	Jun 13, 2016
Publication date	Sep 25, 2018
Grant date	Sep 25, 2018

Abstract

Disclosed is a method for preparing the same. The method for preparing a carrier including ammonium oxidizing bacteria immobilized therein includes: preparing a PVA-alginate mixed solution containing PVA mixed with alginate; adding sludge containing ammonium oxidizing bacteria and sodium bicarbonate (NaHCO 3 ) to the PVA-alginate mixed solution to obtain a foaming-beading solution; and dropping the foaming-beading solution to a saturated boric acid solution to obtain beads including sludge immobilized therein, wherein sodium bicarbonate (NaHCO 3 ) is decomposed to produce carbon dioxide (CO 2 ) which is discharged to the exterior of the beads to form pores in the beads, when the foaming-beading solution is dropped to the saturated boric acid solution to obtain beads including sludge immobilized therein.

First claim

What is claimed is:

1. A method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein, the method comprising:
preparing a polyvinyl alcohol (PVA)-alginate mixed solution containing PVA mixed with alginate;
adding sludge containing ammonium oxidizing bacteria to the PVA-alginate mixed solution;
adding sodium bicarbonate (NaHCO 3 ) to the PVA-alginate mixed solution and mixing the PVA-alginate mixed solution, the sludge containing ammonium oxidizing bacteria, and the sodium bicarbonate (NaHCO 3 ) to obtain a foaming-beading solution; and
dropping the foaming-beading solution to a saturated boric acid solution to obtain beads including the sludge immobilized therein,
wherein, in response to the foaming-beading solution being dropped to the saturated boric acid solution to obtain the beads including the sludge immobilized therein, the sodium bicarbonate (NaHCO 3 ) is decomposed to produce carbon dioxide (CO 2 ) which is discharged to the exterior of the beads to form pores in the beads.

2. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , wherein the foaming-beading solution comprises the sodium bicarbonate (NaHCO 3 ) in an amount of 0.15-1.2% (w/v).

3. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , wherein the foaming-beading solution comprises the sodium bicarbonate (NaHCO 3 ) in an amount of 0.5-0.7% (w/v).

4. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , further comprising:
dipping the beads including the beads immobilized therein in a phosphoric acid solution to increase a mechanical strength.

5. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , further comprising:
providing zeolite to the foaming-beading solution to control specific gravity of the beads.

6. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , wherein the saturated boric acid solution is controlled to have a pH of 3-4.

7. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , further comprising:
dipping the beads comprising the sludge immobilized therein in distilled water to induce swelling of the beads for the discharging of the sodium bicarbonate (NaHCO 3 ) and unreacted alginate remaining in the beads to the exterior of the beads.

8. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , further comprising:
adding solid particles to the foaming-beading solution and mixing the solid particles with the foaming-beading solution,
wherein the solid particles are detached from the beads to form pores at portions where the solid particles are detached.

9. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 8 , wherein the solid particles comprise activated carbon.

10. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 1 , wherein the ammonium oxidizing bacteria comprise anaerobic ammonium oxidizing bacteria or aerobic ammonium oxidizing bacteria.

11. The method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein according to claim 10 , wherein heterotrophic denitrifying bacteria is used to remove nitrite or nitrate in the form of dinitrogen gas.

12. A carrier comprising ammonium oxidizing bacteria immobilized therein obtained by the method as defined in claim 1 .

13. A method for preparing a carrier comprising ammonium oxidizing bacteria immobilized therein, the method comprising:
providing a polyvinyl alcohol (PVA)-alginate mixed solution comprising PVA mixed with alginate;
providing sodium bicarbonate (NaHCO 3 ), zeolite, and sludge comprising ammonium oxidizing bacteria to the PVA-alginate mixed solution;
mixing the NaHCO 3 , the zeolite, the sludge comprising ammonium oxidizing bacteria, and the PVA-alginate mixed solution to obtain a foaming-beading solution; and
dropping the obtained foaming-beading solution to a saturated boric acid solution to form beads including the sludge immobilized therein,
wherein the beads comprise pores formed in response to the NaHCO 3 being decomposed to produce carbon dioxide (CO2).

Assignees

• Korea Inst Sci & Tech

Inventors

• Bae Hyokwan
• Lee Seockheon
• Chung Yunchul
• Jung Jin Young
• Choi Dae Hee
• Lee Dong Ryeol

Classifications

• C02F3/104

  Granular carriers · CPC title

• C12N11/08

  the carrier being a synthetic polymer · CPC title

• C12N11/10 Primary

  the carrier being a carbohydrate · CPC title

• C12N11/04

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• C02F2101/16

  Nitrogen compounds, e.g. ammonia · CPC title