Composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid and their derivatives and method of preparation thereof

Patent metadata

Field	Value
Publication number	US-9962421-B2
Application number	US-201715621078-A
Country	US
Kind code	B2
Filing date	Jun 13, 2017
Priority date	May 26, 2009
Publication date	May 8, 2018
Grant date	May 8, 2018

Abstract

A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent, and a method for use in the preparation of a composition containing caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives. The method includes the steps of pre-concentrating an extract containing the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives and isolating said caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives from said extract by liquid chromatography, wherein the elution activity of said caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives vary depending on the stationary phase and the composition of the mobile phase.

First claim

The invention claimed is:

1. A method for preparing a composition comprising caffeoylshikimic acids and their derivatives, said method comprising:
providing an extract from any part of oil palm;
pre-concentrating said extract containing said caffeoylshikimic acids and their derivatives; and
isolating said caffeoylshikimic acids and their derivatives from said extract by high performance liquid chromatography (HPLC) with a C18 column with 25 length and 10 mm id, using at least one solvent containing 0.1% trifluoroacetic acid and another solvent containing 10/90 0.1% trifluoroacetic acid per acetonitrile for mobile phase gradient, wherein the elution activity of said caffeoylshikimic acids and their derivatives results in a plurality of peaks on said liquid chromatography; wherein the elution activity occurs at 40, 44 and 48 minutes within at least one fraction of the extract, thereby producing at least three peaks by said liquid chromatography.

2. The method of claim 1 , wherein the pre-concentrating step further comprises:
loading a column with the extract,
removing the impurities,
eluting the extract with methanol or ethanol,
concentrating in a rotary evaporator,
adding an amount of cold ethanol, storing at −20° C., centrifuging said mixture, dissolving the precipitate obtained from centrifuging with an amount of water and concentrating said mixture by rotary evaporator at 50° C.

3. The method of claim 2 , wherein the amount of cold ethanol is at least 3 volumes of the extract.

4. The method of claim 2 , wherein the amount of water for dissolving the precipitate is approximately 10 ml.

5. The method of claim 1 , wherein the percentage of purity of the caffeoylshikimic acids and their derivatives obtained upon isolated is within 90 to 95%.

Assignees

• Malaysian Palm Oil Board

Inventors

• Sambandan T G
• Rha Chokyun
• Sinskey Anthony J
• Sambanthamurthi Ravigadevi
• Tan Yew Ai
• P Manickam Kalyana Sundram
• Wahid Mohd Basri

Classifications

• A61K36/889 Primary

  Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto · CPC title