Synergistic selenopeptide formulations for the protection of dermal papilla cells

Patent metadata

Field	Value
Publication number	US-9498423-B2
Application number	US-201213465873-A
Country	US
Kind code	B2
Filing date	May 7, 2012
Priority date	May 7, 2012
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

The present invention discloses selenium peptide based synergistic compositions for the protection (morphology and viable numbers) of dermal papilla cells. The synergistic compositions disclosed in the present invention comprise (a) 1-O-galloyl-β-D-glucose (β-glucogallin) or 1-O-galloyl-β-D-glucose (β-glucogallin) and gallates (b) concentrate of liquid endosperm of Cocos nucifera and (c) selenopeptides.

First claim

We claim:

1. A method of increasing the tolerance of dermal papilla cells to stress signals, the method comprising:
bringing into contact the dermal papilla cells and a formulation comprising a composition; wherein
the composition comprises:
1-O-galloyl-β-D-glucose,
concentrate of liquid endosperm of Cocos nucifera , and
0.001% w/w of a selenopeptide, the selenopeptide being selected from the group consisting of γ-L-glutamyl-selenomethyl-L-selenocysteine, and γ-L-glutamyl-selenomethyl-L-selenomethionine; and

the formulation brings about the effect of maintaining the morphology and numbers of the dermal papilla cells when exposed to stress signals.

2. The method of claim 1 , wherein the composition comprises 0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids.

3. The method of claim 1 , wherein the composition comprises at least 10% w/w of 1-O-galloyl-β-D-glucose.

4. The method of claim 1 , wherein the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenocysteine.

5. The method of claim 1 , wherein the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenomethionine.

6. The method of claim 1 , wherein the composition comprises:
0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids,
at least 10% w/w of 1-O-galloyl-β-D-glucose, and
the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenocysteine.

7. The method of claim 1 , wherein the composition comprises:
0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids,
at least 10% w/w of 1-O-galloyl-β-D-glucose, and
the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenomethionine.

8. The method of claim 1 , wherein the stress signals are UVB radiation levels, and the numbers of live dermal papilla cells are maintained at a level of 95% or more when the dermal papilla cells are exposed to UVB radiation levels are in the range of from 0.648 J/cm 2 to 1.0 J/cm 2 .

9. The method of claim 1 , wherein the stress signals are UVB radiation levels, and the numbers of live dermal papilla cells are maintained at a level of 95% or more when the dermal papilla cells are exposed to UVB radiation levels are in the range of from 0.8 J/cm 2 to 1.0 J/cm 2 .

10. A method of increasing the tolerance of dermal papilla cells to stress signals, the method comprising:
bringing into contact the dermal papilla cells and a formulation comprising a composition; wherein
the composition comprises:
1-O-galloyl-β-D-glucose,
mucic acid gallates,
concentrate of liquid endosperm of Cocos nucifera , and
0.001% w/w of a selenopeptide, the sclenopeptide being selected from the group consisting of γ-L-glutamyl-selenomethyl-L-selenocysteine, and γ-L-glutamyl-selenomethyl-L-selenomethionine; and

the formulation brings about the effect of maintaining the morphology and numbers of the dermal papilla cells when exposed to stress signals.

11. The method of claim 10 , wherein the composition comprises 0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids.

12. The method of claim 10 , wherein the composition comprises at least 10% w/w of 1-O-galloyl-β-D-glucose, and 50% w/w or greater total gallates.

13. The method of claim 10 , wherein the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenocysteine.

14. The method of claim 10 , wherein the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenomethionine.

15. The method of claim 10 , wherein the composition comprises:
0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids,
at least 10% w/w of 1-O-galloyl-β-D-glucose,
50% w/w or greater of total gallates selected from mucic acid 1,4-lactone-5-O-gallate, mucic acid 2-O-gallate, mucic acid 6-methyl ester 2-O-gallate, mucic acid 1-methyl ester 2-O-gallate and ellagic acid, and
the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenocysteine.

16. The method of claim 10 , wherein the composition comprises:
0.5% w/w of the concentrate from the liquid endosperm of Cocos nucifera , the concentrate comprising not less than 40% w/w of total dissolved solids,
at least 10% w/w of 1-O-galloyl-β-D-glucose,
50% w/w or greater of total gallates selected from mucic acid 1,4-lactone-5-O-gallate, mucic acid 2-O-gallate, mucic acid 6-methyl ester 2-O-gallate, mucic acid 1-methyl ester 2-O-gallate and ellagic acid, and
the selenopeptide is γ-L-glutamyl-selenomethyl-L-selenomethionine.

17. The method of claim 10 , wherein the stress signals are UVB radiation levels, and the numbers of live dermal papilla cells are maintained at a level of 95% or more when the dermal papilla cells are exposed to UVB radiation levels are in the range of from 0.648 J/cm 2 to 1.0 J/cm 2 .

18. The method of claim 10 , wherein the stress signals are UVB radiation levels, and the numbers of live dermal papilla cells are maintained at a level of 95% or more when the dermal papilla cells are exposed to UVB radiation levels are in the range of from 0.8 J/cm 2 to 1.0 J/cm 2 .

Assignees

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Sami Labs Ltd

Inventors

• Majeed Muhammed
• Nagabhushanam Kalyanam

Classifications

• A61P39/00

  General protective or antinoxious agents · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P17/00

  Drugs for dermatological disorders · CPC title

• A61P17/16

  Emollients or protectives, e.g. against radiation · CPC title

• A61K8/9794

  Liliopsida [monocotyledons] · CPC title