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Anthropogenic insect-resistant gene and Cry1C toxin idiotype single-chain antibody encoded thereby and application thereof
US9745385B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9745385-B2 |
| Application number | US-201515025805-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 9, 2015 |
| Priority date | Jan 26, 2014 |
| Publication date | Aug 29, 2017 |
| Grant date | Aug 29, 2017 |
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Abstract
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An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a β-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV; the β-type Cry1C toxin idiotype single-chain antibody of the present invention is obtained without animal immunization, has a short preparation period and small amino acid sequence, and is suitable for large-scale in vitro production. The present invention is an entirely new insect-resistant gene resource, and has significant implications for decreasing the various safety risks associated with the widescale use of existing Bt toxins, substituting Bt toxins in the biocontrol of agricultural pests, and reducing the use of pesticides.
First claim
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What is claimed is: 1. An insect-resistant gene, comprising the nucleotide sequence of SEQ ID NO: 1. 2. An anti-Cry1C toxin idiotype single-chain antibody encoded by the insect-resistant gene according to claim 1 , wherein the antibody comprises the amino acid sequence of SEQ ID NO: 2. 3. A prokaryotic vector containing the insect-resistant gene according to claim 1 . 4. An insecticide composition comprising the anti-Cry1C toxin idiotype single-chain antibody according to claim 2 . 5. A method for controlling agriculture pests wherein said method comprises contacting the pests with a pesticidally effective amount of the anti-Cry1C toxin idiotype single-chain antibody according to claim 2 .
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Classifications
Bacillus (G) · CPC title
containing heterocyclic radicals · CPC title
Antagonist effect on antigen, e.g. neutralization or inhibition of binding · CPC title
Single chain antibody (scFv) · CPC title
Human Necessities · mapped topic
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- What does patent US9745385B2 cover?
- An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a β-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has bi…
- Who is the assignee on this patent?
- Jiangsu Acad Agricultural Sci
- What technology area does this patent fall under?
- Primary CPC classification C07K16/4233. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 29 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).